Kozlowski P A, Lynch R M, Patterson R R, Cu-Uvin S, Flanigan T P, Neutra M R
GI Cell Biology Research Laboratory, Department of Medicine, Children's Hospital, Harvard Medical School, Boston, Massachusetts, USA.
J Acquir Immune Defic Syndr. 2000 Aug 1;24(4):297-309. doi: 10.1097/00126334-200008010-00001.
Weck-Cel sponges were examined for suitability as an absorbent material for nontraumatic collection of rectal secretions in humans. Sponges were tested in vitro and determined by quantitative enzyme-linked immunosorbent assay (ELISA) to be capable of releasing 100% of absorbed albumin and all immunoglobulin subtypes after treatment with detergent-supplemented buffer. Protein composition in rectal secretions collected from normal women with dry sponges (DS) or with sponges previously softened by moistening with saline (MS) was subsequently compared. DS secretions showed evidence of contamination with blood and interstitial fluid-derived albumin, immunoglobulin G (IgG), and monomeric IgA. MS secretions appeared to represent local mucosal secretions more accurately because they contained negligible blood, a greater percentage of secretory IgA within the total IgA, and both lower albumin/IgG ratios and more dramatic alterations in IgG subclass distribution compared with corresponding serum. Anti-HIV IgG, IgM, IgA, and antibodies with secretory component could be demonstrated by ELISA in rectal secretions collected with moist sponges from 8 of 8, 1 of 8, 5 of 8, and 3 of 8 HIV-infected women, respectively. The data show that Weck-Cel sponges, if premoistened, can be used to collect rectal fluids nontraumatically and to obtain quantitative information about concentrations of immunoglobulins and specific antibodies on rectal mucosal surfaces.
对Weck-Cel海绵进行了检测，以确定其是否适合作为一种吸收材料，用于非创伤性收集人体直肠分泌物。海绵在体外进行了测试，并通过定量酶联免疫吸附测定（ELISA）确定，在用添加了去污剂的缓冲液处理后，能够释放100%吸收的白蛋白和所有免疫球蛋白亚型。随后比较了用干燥海绵（DS）或预先用盐水浸湿软化的海绵（MS）从正常女性收集的直肠分泌物中的蛋白质组成。DS分泌物显示有血液和间质液来源的白蛋白、免疫球蛋白G（IgG）和单体IgA污染的迹象。MS分泌物似乎更准确地代表了局部黏膜分泌物，因为与相应血清相比，它们含有的血液可忽略不计，总IgA中分泌型IgA的百分比更高，白蛋白/IgG比值更低，且IgG亚类分布的变化更显著。通过ELISA可以在分别从8名感染HIV的女性中的8名、1名、5名和3名女性用湿海绵收集的直肠分泌物中检测到抗HIV IgG、IgM、IgA和带有分泌成分的抗体。数据表明，Weck-Cel海绵如果预先浸湿，可以用于非创伤性收集直肠液，并获得有关直肠黏膜表面免疫球蛋白和特异性抗体浓度的定量信息。
