Loï C, Chardigny J M, Almanza S, Leclere L, Ginies C, Sébédio J L
INRA, Unité de Nutrition Lipidique, BP 86510-21605 Dijon, France and. INRA, Laboratoire de Recherche sur les Arômes, 21000 Dijon, France.
J Nutr. 2000 Oct;130(10):2550-5. doi: 10.1093/jn/130.10.2550.
To study the influence on lipid metabolism and platelet aggregation of the fatty acid isomerization that occurs during heat treatment, weanling rats were fed for 8 wk a diet enriched with 5% isomerized (experimental group) or normal (control group) canola oil. Geometrical isomers of alpha-linolenic acid representing 0.2 g/100 g of the experimental diet were incorporated into liver, platelets, aorta and heart, at the expense of their cis homologue and of 18:2(n-6). The major isomer, 9c,12c,15t-18:3, was also metabolized to 5c,8c,11c,14c,17t-20:5 and to an unknown compound, found in liver, platelets and aorta, which has been identified tentatively as 7c, 10c,13c,16c,19t-22:5. The greater 20:4(n-6)/18:2(n-6) ratio in the liver, platelets and heart of the experimental group than the control group indicated an enhancement of desaturation activities. This induced a higher content of long-chain (n-6) fatty acids in the experimental group. Platelet aggregation tended to be slightly higher (P: = 0.065) in the experimental group. We conclude that 0.2 g of trans isomers of alpha-linolenic acid per 100 g of diet was sufficient to be incorporated and metabolized, thus altering the fatty acid profile of rat tissues.
为研究热处理过程中发生的脂肪酸异构化对脂质代谢和血小板聚集的影响，将断乳大鼠用富含5%异构化（实验组）或正常（对照组）菜籽油的饲料喂养8周。每100克实验饲料中含0.2克α-亚麻酸几何异构体，它们被纳入肝脏、血小板、主动脉和心脏，取代了其顺式同系物和18:2(n-6)。主要异构体9c,12c,15t-18:3也被代谢为5c,8c,11c,14c,17t-20:5以及一种未知化合物，该化合物在肝脏、血小板和主动脉中被发现，暂被鉴定为7c,10c,13c,16c,19t-22:5。实验组肝脏、血小板和心脏中的20:4(n-6)/18:2(n-6)比值高于对照组，表明去饱和活性增强。这使得实验组中长链(n-6)脂肪酸含量更高。实验组的血小板聚集倾向略高（P = 0.065）。我们得出结论，每100克饲料中0.2克α-亚麻酸反式异构体足以被纳入和代谢，从而改变大鼠组织的脂肪酸谱。
