Pinon J M, Sulahian A, Remy G, Dropsy G
Am J Trop Med Hyg. 1979 Mar;28(2):318-24.
The comparative sensitivity, specificity and rapidity of immunoelectrodiffusion (IED) on cellulose acetate membranes and of immunoelectrophoresis (IEP) on agarose gel, were evaluated in the diagnosis of hydatidosis. Pooled crude fluid from several cattle hydatis cysts was used as antigen in tests on 1,750 non-hydatis and 400 hydatis sera obtained from patients with hepatic, pulmonary, splenic, peritoneal and cerebral hydatidosis before and after surgery. Coupled to a specific human reference serum for arc 5, IED shows a specificity comparable to that of IEP but its sensitivity is slightly higher and the amount of antigen needed is very small. The appearance of a typical "gloved finger" pattern in sera from patients with ruptured cysts emphasizes the interest of quick results (3 hours) obtained by this method. In order to increase the sensitivity of IED and to define the class of immunoglobulins involved in the antigen-antibody reaction, we have coupled this method to an enzymatic technique. The immune complexes precipitated by IED were treated with peroxidase-labelled antibodies specific to each class of human immunoglobulins. The specificity of this enzyme-linked immunoelectrodiffusion assay (ELIEDA) permits one to follow the immunologic evolution of hydatidosis and to identify IgM in ruptured hepatic cysts and IgA in pulmonary cysts.
在包虫病的诊断中，对醋酸纤维素膜上的免疫电扩散（IED）和琼脂糖凝胶上的免疫电泳（IEP）的比较敏感性、特异性和快速性进行了评估。从几个牛包虫囊肿中收集的混合粗液用作抗原，对1750份非包虫病血清和400份包虫病血清进行检测，这些血清来自肝、肺、脾、腹膜和脑包虫病患者手术前后。与针对弧5的特异性人类参考血清相结合，IED显示出与IEP相当的特异性，但其敏感性略高，所需抗原量非常少。囊肿破裂患者血清中典型的“手套指”模式的出现强调了通过该方法获得快速结果（3小时）的重要性。为了提高IED的敏感性并确定抗原-抗体反应中涉及的免疫球蛋白类别，我们将该方法与一种酶技术相结合。用针对每类人类免疫球蛋白的过氧化物酶标记抗体处理由IED沉淀的免疫复合物。这种酶联免疫电扩散测定法（ELIEDA）的特异性使人们能够追踪包虫病的免疫演变，并在破裂的肝囊肿中鉴定IgM，在肺囊肿中鉴定IgA。
