ELIEDA (enzyme-linked-immuno-electro-diffusion-assay): application of a combined immunoelectrodiffusion and immunoenzyme method to the study of immune response in parasitic infections.

The sensitivity of the technique of immunoelectrodiffusion (IED) has been enhanced by combining it with an enzyme method; this also enabled the class of immunoglobulins involved in the reactions to be determined by treating the precipitated immune complexes with enzyme-labelled class-specific antisera. The method has been employed in studies of the immunology of parasitic infections and these confirmed its sensitivity and specificity. ELIEDA provides a valuable tool for analysing responses to complex immunogens.