红细胞膜脂肪酸组成是否是骨骼肌膜脂肪酸组成的有效指标？

Is the erythrocyte membrane fatty acid composition a valid index of skeletal muscle membrane fatty acid composition?

作者信息

Di Marino L, Maffettone A, Cipriano P, Sacco M, Di Palma R, Amato B, Quarto G, Riccardi G, Rivellese A A

机构信息

Department of Clinical and Experimental Medicine, Federico II University Medical School, Naples, Italy.

出版信息

Metabolism. 2000 Sep;49(9):1164-6. doi: 10.1053/meta.2000.8616.

DOI:10.1053/meta.2000.8616

PMID:11016898

Abstract

Recent studies suggest that insulin sensitivity is related to the fatty acid composition of phospholipids in skeletal muscle (SM) membranes. Since it is difficult to obtain muscle biopsies, it may be useful to have information on the fatty acid composition using more accessible cells such as erythrocytes. This would be possible only if the composition of erythrocyte and muscle membranes are very similar. Since no comparative data are available, we evaluated the phospholipid fatty acid composition of erythrocyte and SM membranes in 16 individuals, 10 nondiabetics (male to female ratio, 4:6; age, 50 +/- 11 years; body mass index, 27 +/- 5 kg/m2; mean +/- SD) and 6 type 2 diabetic patients (male to female ratio, 2:4; age, 64 +/- 5 years; body mass index, 27 +/- 4 kg/m2). All patients underwent abdominal surgery, during which a biopsy of the abdominal rectus muscle (50 to 100 mg) was obtained. Erythrocyte and SM phospholipid fatty acids were extracted and then methylated; the methyl fatty acids were separated and quantified by gas chromatography. Compared with erythrocyte membranes, muscle membranes showed a significantly higher proportion of omega-6 polyunsaturated fatty acid ([PUFA] 43.0% +/- 3.1% v29.7% +/- 1.6%, P < .001) and lower saturated fatty acid ([SFA] 41.1% +/- 1.5% v 43.4% +/- 1.2%, P < .001), monounsaturated fatty acid ([MUFA] 11.5% +/- 1.7% v 20.0% +/- 1.9%, P < .001), and omega-3 PUFA (3.8% +/- 0.6% v 7.4% +/- 1.0%, P < .001). The greatest increase involved linoleic acid (26.9% +/- 2.8% v 10.3% +/- 1.6%, P < .001), whereas lignoceric acid (0.8% +/- 0.2% v 5.0% +/- 0.6%, P < .001) and oleic acid (10.4% +/- 1.6% v 13.5% +/- 1.3%, P < .001) were significantly lower. These results show that erythrocyte and muscle membrane phospholipid fatty acids are significantly different. Therefore, data on SM membranes cannot be extrapolated on the basis of measures of erythrocyte phospholipid fatty acid composition.

摘要

近期研究表明，胰岛素敏感性与骨骼肌（SM）细胞膜中磷脂的脂肪酸组成有关。由于获取肌肉活检样本较为困难，因此利用更容易获取的细胞（如红细胞）来获取脂肪酸组成信息可能会有所帮助。只有当红细胞膜和肌肉膜的组成非常相似时，才有可能实现这一点。由于尚无比较数据，我们评估了16名个体的红细胞膜和SM膜的磷脂脂肪酸组成，其中10名非糖尿病患者（男女比例为4:6；年龄50±11岁；体重指数27±5kg/m²；平均值±标准差）和6名2型糖尿病患者（男女比例为2:4；年龄64±5岁；体重指数27±4kg/m²）。所有患者均接受了腹部手术，术中获取了腹直肌活检样本（50至100mg）。提取红细胞和SM磷脂脂肪酸，然后进行甲基化；通过气相色谱法分离并定量甲基脂肪酸。与红细胞膜相比，肌肉膜中ω-6多不饱和脂肪酸的比例显著更高（[PUFA]43.0%±3.1%对29.7%±1.6%，P<.001），而饱和脂肪酸（[SFA]41.1%±1.5%对43.4%±1.2%，P<.001）、单不饱和脂肪酸（[MUFA]11.5%±1.7%对20.0%±1.9%，P<.001）和ω-3多不饱和脂肪酸（3.8%±0.6%对7.4%±1.0%，P<.001）的比例显著更低。增加最多的是亚油酸（26.9%±2.8%对10.3%±1.6%，P<.001），而二十四烷酸（0.8%±0.2%对5.0%±0.6%，P<.001）和油酸（10.4%±1.6%对13.5%±1.3%，P<.001）显著更低。这些结果表明，红细胞膜和肌肉膜的磷脂脂肪酸存在显著差异。因此，不能根据红细胞磷脂脂肪酸组成的测量结果来推断SM膜的数据。