Sanchez-Vives M V, McCormick D A
Section of Neurobiology, Yale University School of Medicine, 333 Cedar Street, New Haven, Connecticut 06510, USA.
Nat Neurosci. 2000 Oct;3(10):1027-34. doi: 10.1038/79848.
The neocortex generates periods of recurrent activity, such as the slow (0.1-0.5 Hz) oscillation during slow-wave sleep. Here we demonstrate that slices of ferret neocortex maintained in vitro generate this slow (< 1 Hz) rhythm when placed in a bathing medium that mimics the extracellular ionic composition in situ. This slow oscillation seems to be initiated in layer 5 as an excitatory interaction between pyramidal neurons and propagates through the neocortex. Our results demonstrate that the cerebral cortex generates an 'up' or depolarized state through recurrent excitation that is regulated by inhibitory networks, thereby allowing local cortical circuits to enter into temporarily activated and self-maintained excitatory states. The spontaneous generation and failure of this self-excited state may account for the generation of a subset of cortical rhythms during sleep.
新皮层会产生周期性的反复活动，比如慢波睡眠期间的慢（0.1 - 0.5赫兹）振荡。我们在此证明，雪貂新皮层切片在体外培养时，置于模拟原位细胞外离子组成的浴液中会产生这种慢（<1赫兹）节律。这种慢振荡似乎起始于第5层，是锥体神经元之间的一种兴奋性相互作用，并通过新皮层进行传播。我们的结果表明，大脑皮层通过由抑制性网络调节的反复兴奋产生一种“上行”或去极化状态，从而使局部皮层回路进入暂时激活且自我维持的兴奋状态。这种自激状态的自发产生和失败可能解释了睡眠期间一部分皮层节律的产生。
