Pettersson B, Eriksson K E
STFI, Swedish Pulp and Paper Research Institute, SE-114 86 Stockholm, Sweden.
Anal Biochem. 2000 Oct 15;285(2):220-4. doi: 10.1006/abio.2000.4776.
This is a new technique to assay virtually any endoglycanase activity where enough polysaccharide material is available to allow for production of the amorphous, dyed beads used as substrates. It allows for a direct comparison of endoglycanase activities between laboratories since dyed beads from at least the most common polysaccharides such as cellulose, xylan, mannan, and chitin are now under development and will soon be commercially available; cellulose beads already are. It is a very sensitive technique and enzyme activities can be measured using a nonsophisticated spectrophotometer.
这是一种几乎可以测定任何内切聚糖酶活性的新技术，前提是有足够的多糖材料来制备用作底物的无定形染色珠子。由于至少纤维素、木聚糖、甘露聚糖和几丁质等最常见多糖的染色珠子正在开发中，很快将上市销售（纤维素珠子已经有了），因此该技术能够直接比较不同实验室的内切聚糖酶活性。这是一种非常灵敏的技术，使用普通的分光光度计就能测量酶活性。
