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一种β-D-甘露聚糖酶的简易测定方法。

A simple assay procedure for beta-D-mannanase.

作者信息

McCleary B V

出版信息

Carbohydr Res. 1978 Nov;67(1):213-21. doi: 10.1016/s0008-6215(00)83743-x.

DOI:10.1016/s0008-6215(00)83743-x
PMID:213193
Abstract

A simple assay procedure for beta-D-mannanase enzyme has been developed which employs carob D-galacto-D-mannan dyed with Remazolbrilliant Blue. Additionally, the procedure is quantitative, relatively sensitive, and highly specific for beta-D-mannanase enzyme. It can be readily used for the determination of beta-D-mannanase activity in crude enzyme preparations and column-chromatography eluates.

摘要

已开发出一种用于β-D-甘露聚糖酶的简单测定方法，该方法采用用雷马素亮蓝染色的角豆D-半乳糖-D-甘露聚糖。此外，该方法是定量的，相对灵敏，且对β-D-甘露聚糖酶具有高度特异性。它可轻松用于测定粗酶制剂和柱色谱洗脱液中的β-D-甘露聚糖酶活性。

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Abscisic Acid Is an Endogenous Inhibitor in the Regulation of Mannanase Production by Isolated Lettuce (Lactuca sativa cv Grand Rapids) Endosperms.
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