通过高效液相色谱法分离和定量磷脂及溶血磷脂
Separation and quantitation of phospholipids and lysophospholipids by high-performance liquid chromatography.
作者信息
Lesnefsky E J, Stoll M S, Minkler P E, Hoppel C L
机构信息
Department of Medicine, Case Western Reserve University, Cleveland, Ohio 44106, USA.
出版信息
Anal Biochem. 2000 Oct 15;285(2):246-54. doi: 10.1006/abio.2000.4783.
We describe a comprehensive approach to the separation, quantitation, and characterization of phospholipids and lysophospholipids present in complex biological samples. The central feature is a normal-phase HPLC separation of individual phospholipid and lysophospholipid classes. In this single chromatographic step, phospholipids and lysophospholipids are separated and recovered for quantitation by organic phosphate assay and characterization by acyl-group composition. Recovery of phospholipids and lysophospholipids from HPLC averages 80-90%. Isolated phospholipid and lysophospholipid fractions are available for separation of individual molecular species by second-dimension reverse-phase HPLC and characterization of individual molecular species by mass spectrometry.
我们描述了一种用于分离、定量和表征复杂生物样品中存在的磷脂和溶血磷脂的综合方法。其核心特点是对各个磷脂和溶血磷脂类别进行正相高效液相色谱分离。在这一单一色谱步骤中，磷脂和溶血磷脂被分离并回收，通过有机磷酸盐测定进行定量，并通过酰基组成进行表征。从高效液相色谱中回收磷脂和溶血磷脂的平均回收率为80 - 90%。分离得到的磷脂和溶血磷脂级分可用于通过二维反相高效液相色谱分离各个分子种类，并通过质谱对各个分子种类进行表征。
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