Peterson Brianna L, Cummings Brian S
Department of Pharmaceutical and Biomedical Sciences, The University of Georgia, Athens, 30602-2352, USA.
Biomed Chromatogr. 2006 Mar;20(3):227-43. doi: 10.1002/bmc.563.
Phospholipids are important constituents of all living cell membranes. Lipidomics is a rapidly growing field that provides insight as to how specific phospholipids play roles in normal physiological and disease states. There are many analytical methods available for the qualitative and quantitative determination of phospholipids. This review provides a summary of the methods that were historically used such as thin layer chromatography, gas chromatography and high-performance liquid chromatography. In addition, an introduction to applications of interfacing these traditional chromatographic techniques with mass spectrometry is provided.
磷脂是所有活细胞膜的重要组成部分。脂质组学是一个快速发展的领域，它能让我们了解特定磷脂在正常生理状态和疾病状态中所起的作用。有许多分析方法可用于磷脂的定性和定量测定。本综述总结了历史上使用的方法，如薄层色谱法、气相色谱法和高效液相色谱法。此外，还介绍了将这些传统色谱技术与质谱联用的应用。
