Nexo E, Christensen A L, Petersen T E, Fedosov S N
Department of Clinical Biochemistry, AKH, Aarhus University Hospital, Norrebrogade 44, DK-8000 Aarhus C, Denmark.
Clin Chem. 2000 Oct;46(10):1643-9.
Transcobalamin is essential for the cellular internalization of cobalamin. Methods to quantify the unsaturated protein are available, but few attempts have been made to develop methods to quantify the sum of unsaturated and cobalamin saturated transcobalamin.
gamma-Globulins from two polyclonal rabbit antibodies against recombinant human transcobalamin were used as capture and detection antibodies, and recombinant human transcobalamin was used as calibrator in an ELISA design.
The ELISA is specific for transcobalamin and has a detection limit of <1.6 pmol/L. The imprecision (CV) is 4-6% for mean concentrations of 13-70 pmol/L. The central 95% interval for serum from healthy blood donors (n = 77) was approximately 600-1500 pmol/L and showed limited variation with age and sex. No correlation was observed between the marker of acute phase reaction, C-reactive protein, and transcobalamin in plasma.
The ELISA measures total transcobalamin in serum and thus can be used for measurement of transcobalamin in patients treated with cobalamin.
转钴胺素对于钴胺素的细胞内化至关重要。已有定量不饱和蛋白的方法，但很少有人尝试开发定量不饱和及钴胺素饱和转钴胺素总和的方法。
在酶联免疫吸附测定（ELISA）设计中，使用来自两种针对重组人转钴胺素的多克隆兔抗体的γ球蛋白作为捕获抗体和检测抗体，并使用重组人转钴胺素作为校准物。
该ELISA对转钴胺素具有特异性，检测限<1.6 pmol/L。对于13 - 70 pmol/L的平均浓度，不精密度（CV）为4 - 6%。健康献血者（n = 77）血清的中心95%区间约为600 - 1500 pmol/L，且随年龄和性别变化有限。在急性期反应标志物C反应蛋白与血浆中转钴胺素之间未观察到相关性。
该ELISA可测定血清中的总转钴胺素，因此可用于测定接受钴胺素治疗患者的转钴胺素。
