Wu J P, Kuo J S, Liu Y L, Tzeng S F
Department of Research and Education, Taichung Veterans General Hospital, 40705, Taichung city, Taiwan.
Neurosci Lett. 2000 Oct 13;292(3):203-6. doi: 10.1016/s0304-3940(00)01472-5.
Little is known about the response of neural progenitors to inflammation following injuries of the central nervous system. In combination with bromodeoxyuridine (BrdU) intraperitoneally (i.p.) injected, tumor necrosis factor-alpha (TNF-alpha), a proinflammatory cytokine that increased ED1+ activated microglia/macrophage population at injured sites, was administrated into adult rat brains. No difference in the immunostaining for proliferating cell nuclear antigen (PCNA) was observed in the subventricular/ventricular zone (SVZ/VZ) between TNF-alpha injected sites and controls. However, BrdU+ cells were apparently observed in the SVZ/VZ proximal to TNF-alpha injected site, and the number of BrdU+ cells increased at 6 and 24 h post injection. Since cell apoptosis was rarely found in the SVZ/VZ after TNF-alpha injection, these observations suggest that the diffusible TNF-alpha may directly and/or indirectly modulate the proliferation of neural progenitors.
关于中枢神经系统损伤后神经祖细胞对炎症的反应，人们了解甚少。将促炎细胞因子肿瘤坏死因子-α（TNF-α）注入成年大鼠脑内，该因子可增加损伤部位ED1 + 活化小胶质细胞/巨噬细胞数量，同时腹腔内注射溴脱氧尿苷（BrdU）。在注射TNF-α的部位与对照部位的脑室下/脑室区（SVZ/VZ），未观察到增殖细胞核抗原（PCNA）免疫染色的差异。然而，在靠近注射TNF-α部位的SVZ/VZ中明显观察到BrdU + 细胞，且注射后6小时和24小时BrdU + 细胞数量增加。由于注射TNF-α后在SVZ/VZ中很少发现细胞凋亡，这些观察结果表明，可扩散的TNF-α可能直接和/或间接调节神经祖细胞的增殖。
