[通过二维电泳分析少量多肽：在蛋白质表征中的应用]
[Analysis of a small quatity of polypeptides by two-dimensional electrophoresis: application to characterization of proteins].
作者信息
Portier C
出版信息
Biochimie. 1975;57(5):539-44. doi: 10.1016/s0300-9084(75)80133-7.
PMID:1101971
Abstract
A new two-dimensional electrophoresis is described. The first electrophoresis is performed in a 10 p. cent gel (1 X 110 mm) in the presence of 7 M urea; the migration in the second dimension proceeds on a 20 p. cent polyacrylamide sheet (160 X 110 X 1 mm) in the presence of sodium dodecyl sulphate. This system resolves most of the polypeptides obtained by treating a very small amount (about 100 mug) of proteins with cyanogene bromide. Macromolecules can thus be quickly and easily characterized.
摘要
本文描述了一种新的二维电泳方法。第一次电泳在含有7M尿素的10%凝胶（1×110mm）中进行；第二次电泳在含有十二烷基硫酸钠的20%聚丙烯酰胺薄板（160×110×1mm）上进行。该系统可分离通过用溴化氰处理极少量（约100微克）蛋白质而得到的大多数多肽。因此，可以快速且容易地对大分子进行表征。
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