化学裂解方法在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳中对神经丝三联体蛋白条带进行肽图谱分析的应用。
Applications of chemical cleavage procedures to the peptide mapping of neurofilament triplet protein bands in sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
作者信息
Mahboub S, Richard C, Delacourte A, Han K K
出版信息
Anal Biochem. 1986 Apr;154(1):171-82. doi: 10.1016/0003-2697(86)90511-7.
PMID:3085540
Abstract
A procedure for examining possible sequence homology in the triplet neurofilament proteins using a sodium dodecyl sulfate-polyacrylamide gel electrophoresis system is described. Five different chemical reagents (cyanogen bromide, BNPS-skatole, hydroxylamine, formic acid, and nitrothiocyanobenzoic acid) have been used for peptide mapping studies. Potential applications of this technique are discussed.
摘要
本文描述了一种使用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳系统检测三联体神经丝蛋白中可能的序列同源性的方法。已使用五种不同的化学试剂（溴化氰、BNPS-粪臭素、羟胺、甲酸和硝基硫氰基苯甲酸）进行肽图谱研究。讨论了该技术的潜在应用。
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