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在组织学分类为唯支持细胞的患者睾丸组织中鉴定减数分裂和减数分裂后基因表达。

Identification of meiotic and postmeiotic gene expression in testicular tissue of patients histologically classified as Sertoli cell only.

作者信息

Patrizio P, Ricci S M, Tomaszewski J E, Hecht N B

机构信息

Center for Research on Reproduction and Women's Health, University of Pennsylvania Medical Center, Philadelphia, Pennsylvania, USA.

出版信息

Fertil Steril. 2000 Oct;74(4):785-90. doi: 10.1016/s0015-0282(00)01515-6.

DOI:10.1016/s0015-0282(00)01515-6
PMID:11020524
Abstract

OBJECTIVE

To determine whether meiotic and postmeiotic germ cell gene products could be detected in biopsy specimen from patients with Sertoli cell only (SCO) and maturation arrest.

DESIGN

Prospective clinical study.

SETTING

University-based departments and laboratories.

PATIENT(S): Nine patients, seven with nonobstructive azoospermia (12 biopsies) and two with obstructive azoospermia (controls) (2 biopsies).

INTERVENTION(S): Specimens were divided into three parts: IVF laboratory, histology, and molecular analysis. Germ cell-specific messenger RNAs (mRNAs) were detected by extracting total RNA for Northern blotting or reverse transcription-polymerase chain reaction.

MAIN OUTCOME MEASURE(S): Detection of meiotic (lactate dehydrogenase C4) and postmeiotic (transition protein 1 and protamine 1 and 2) gene expression and correlation with histologic and IVF laboratory findings.

RESULT(S): The IVF laboratory identified spermatozoa in 3 of 14 biopsies (controls and severe hypospermatogenesis). Histologically, 6 of 14 biopsies (43%) were diagnosed as SCO, 4 (29%) maturation arrest, 2 (14%) severe hypospermatogenesis, and 2 normal. Molecular analysis showed mRNA for meiotic and postmeiotic genes in 12 of 14 biopsies (86%) (P =. 006), of which 4 (67%) in SCO and 3 (75%) in maturation arrest.

CONCLUSION(S): Differentiated germ cells are present in biopsies of men histologically diagnosed as SCO. Absence of these molecular markers strengthens the histologic diagnosis and helps the physician in counseling the infertile couple.

摘要

目的

确定在唯支持细胞(SCO)和成熟停滞患者的活检标本中能否检测到减数分裂及减数分裂后生殖细胞基因产物。

设计

前瞻性临床研究。

地点

大学附属医院科室及实验室。

患者

9例患者,7例非梗阻性无精子症(12次活检),2例梗阻性无精子症(对照)(2次活检)。

干预措施

标本分为三部分:体外受精实验室、组织学和分子分析。通过提取总RNA进行Northern印迹或逆转录聚合酶链反应检测生殖细胞特异性信使核糖核酸(mRNA)。

主要观察指标

检测减数分裂(乳酸脱氢酶C4)和减数分裂后(过渡蛋白1、鱼精蛋白1和2)基因表达,并与组织学及体外受精实验室结果进行相关性分析。

结果

体外受精实验室在14次活检中的3次(对照及严重生精低下)发现精子。组织学检查中,14次活检中有6次(43%)诊断为SCO,4次(29%)成熟停滞,2次(14%)严重生精低下,2次正常。分子分析显示,14次活检中有12次(86%)检测到减数分裂和减数分裂后基因的mRNA(P = 0.006),其中SCO中有4次(67%),成熟停滞中有3次(75%)。

结论

组织学诊断为SCO的男性活检标本中存在分化的生殖细胞。这些分子标志物的缺失强化了组织学诊断,并有助于医生为不育夫妇提供咨询。

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