Song G J, Lee H, Park Y, Lee H J, Lee Y S, Seo J T, Kang I S
Samsung Cheil Hospital and Women's Health Care Center, Sungkyunkwan University School of Medicine, Seoul, Korea, South Korea.
Fertil Steril. 2000 Jun;73(6):1104-8. doi: 10.1016/s0015-0282(00)00520-3.
To analyze the expression pattern of testis-specific genes of patients with various spermatogenic defects and their usefulness as a molecular marker to predict the presence of testicular spermatozoa in patients with nonobstructive azoospermia undergoing IVF.
Prospective, controlled study.
Hospital-based infertility research laboratory.
PATIENT(S): Fifty-eight men with azoospermia or severe oligozoospermia.
INTERVENTION(S): Testicular biopsy was done in the patients with obstructive or nonobstructive azoospermia, including Sertoli cell-only syndrome, maturation arrest, severe hypospermatogenesis, and normal spermatogenesis.
MAIN OUTCOME MEASURE(S): Reverse transcriptase polymerase chain reaction was performed using 1 microgram of total RNA extracted from testicular tissues. Three pairs of primers were used for amplification of male germ cell-specific genes (DAZ, transcribed in male germ cells; PGK2, in late spermatocytes and spermatids; protamine-2, in spermatids) as molecular markers. Testicular sperm was obtained by multiple testicular sperm extraction.
RESULT(S): The DAZ, PGK2, and protamine-2 genes were expressed in 38, 30, and 21 of the 43 patients with nonobstructive azoospermia, respectively. Testicular spermatozoa were successfully extracted in 4 of 43 patients with nonobstructive azoospermia with the use of multiple testicular sperm extraction. Detection of protamine-2 transcripts predicted the presence or absence of spermatozoa in the testicular tissue in 39 of 43 patients (91%).
CONCLUSION(S): Expression of the protamine-2 gene may be a useful molecular marker to predict the presence of testicular sperm in patients with nonobstructive azoospermia.
分析各种生精缺陷患者睾丸特异性基因的表达模式，及其作为分子标志物预测接受体外受精的非梗阻性无精子症患者睾丸精子存在情况的效用。
前瞻性对照研究。
医院不孕不育研究实验室。
58名无精子症或严重少精子症男性。
对梗阻性或非梗阻性无精子症患者进行睾丸活检，包括唯支持细胞综合征、成熟障碍、严重精子发生低下和正常精子发生。
使用从睾丸组织中提取的1微克总RNA进行逆转录聚合酶链反应。使用三对引物扩增男性生殖细胞特异性基因（DAZ，在男性生殖细胞中转录；PGK2，在晚期精母细胞和精子细胞中；鱼精蛋白-2，在精子细胞中）作为分子标志物。通过多次睾丸精子提取获取睾丸精子。
在43例非梗阻性无精子症患者中，DAZ、PGK2和鱼精蛋白-2基因分别在38例、30例和21例中表达。在43例非梗阻性无精子症患者中，有4例通过多次睾丸精子提取成功提取到睾丸精子。检测鱼精蛋白-2转录本在43例患者中的39例（91%）中预测了睾丸组织中精子的存在与否。
鱼精蛋白-2基因的表达可能是预测非梗阻性无精子症患者睾丸精子存在情况的有用分子标志物。
