胃蛋白酶消化的马抗体在定量免疫电泳中的应用。
Application of pepsin-digested horse antibodies for quantitative immunoelectrophoresis.
作者信息
Péterfy F, Varró R, Fátrai Z
出版信息
Clin Chim Acta. 1975 Dec 1;65(2):235-9. doi: 10.1016/0009-8981(75)90113-8.
The majority of the precipitating antibodies in hyperimmune horse serum belong to the beta globulins, as demonstrated by reversed immunoelectrophoresis. As these antibodies migrate in agarose gel during electrophoresis in conventional pH = 8.6-8.9 buffers, horse antiserum cannot be used satisfactorily for quantitative immunoelectrophoresis. On pepsin digestion of horse antiserum F(ab)' 2 fragments are generated which migrate with the gamma globulins. This cleaved product works well in quantitative immunoelectrophoretic techniques.
反向免疫电泳证明，超免疫马血清中的大多数沉淀抗体属于β球蛋白。由于这些抗体在常规pH = 8.6 - 8.9缓冲液中进行电泳时会在琼脂糖凝胶中迁移，马抗血清不能令人满意地用于定量免疫电泳。对马抗血清进行胃蛋白酶消化会产生F(ab)' 2片段，这些片段与γ球蛋白一起迁移。这种裂解产物在定量免疫电泳技术中效果良好。
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