多发性骨髓瘤患者循环白细胞介素-10（IL-10）与白细胞介素-6（IL-6）类细胞因子（IL-6、白细胞介素-11（IL-11）、抑瘤素M（OSM））及可溶性白细胞介素-6（IL-6）受体（sIL-6R）之间的关系

Relationship between circulating interleukin-10 (IL-10) with interleukin-6 (IL-6) type cytokines (IL-6, interleukin-11 (IL-11), oncostatin M (OSM)) and soluble interleukin-6 (IL-6) receptor (sIL-6R) in patients with multiple myeloma.

作者信息

Urbańska-Ryś H, Wiersbowska A, Stepień H, Robak T

机构信息

Department of Haematology, Medical University of L/eodez##, Copernicus Hospital, 93-513 Lódź, Pabianicka 62, Poland.

出版信息

Eur Cytokine Netw. 2000 Sep;11(3):443-51.

DOI:

PMID:11022130

Abstract

We investigated the serum concentration of the interleukin-10 (IL-10), along with cytokines of interleukin-6 (IL-6) family (IL-6, IL-11 and oncostatin M - OSM), as well as soluble receptor for IL-6 (sIL-6R), in 121 patients with multiple myeloma (MM) and 28 healthy subjects. We studied the interactions between IL-10 and other cytokines, and the receptor. The correlation between IL-10 and some clinical and laboratory parameters associated with the disease activity were also analysed. The IL-10 was detectable in all patients with multiple myeloma and in all controls. The IL-10 concentration was significantly increased in myeloma patients compared with healthy persons (mean - 7.09 and 2.1 pg/ml, respectively) (p = 0.008). The level of IL-10 correlated positively with the advanced stage of disease estimated according to the Salmon and Durie classification (I versus III stage - p = 0.03). Higher values of IL-10 were found in patients with the light chain disease, hypercalcaemia, and correlated with the elevated concentrations of C-reactive protein (CRP). IL-6 was detected in 117 of the 121 patients and in all controls. The concentration of IL-6 was statistically increased in MM patients compared with control group (mean - 16.06 and 4.49 pg/ml, respectively) (p = 0.01). We found a positive correlation between IL-10 and IL-6 serum levels in MM patients. The relationship, expressed as Spearman's rank sum coefficient (rho = 0.249, p = 0.006) was significant. IL-11 was detected in 26 of the 121 MM patients and in 3 of the 28 healthy subjects at the mean concentration of 1.2 and 0.6 pg/ml respectively (p > 0.05). OSM was at detectable levels in 51 of the 121 patients and in only 4 of the 28 controls (mean - 3.84 and 0.1 pg/ml, p = 0. 002). The correlation between IL-10 and IL-11 levels in MM patients was not significant, but there was a strong statistical correlation between IL-10 and OSM concentrations (rho= 0.327, p = 0.0002). The serum concentration of sIL-6R was measurable in all patients and all controls (mean - 66.00 and 39.57 ng/ml respectively), but the difference between these groups was not significant. We found significant, positive correlation between the levels of IL-10 and sIL-6R (rho= 0.233, p = 0.01). In conclusion, we state that the serum concentrations of IL-10, IL-6, OSM and sIL-6R in MM patients may be a useful markers for the evaluation of the disease activity.

摘要

我们检测了121例多发性骨髓瘤（MM）患者和28名健康受试者血清中白细胞介素10（IL-10）的浓度，以及白细胞介素6（IL-6）家族细胞因子（IL-6、IL-11和抑瘤素M - OSM）和IL-6可溶性受体（sIL-6R）的浓度。我们研究了IL-10与其他细胞因子及受体之间的相互作用。还分析了IL-10与一些与疾病活动相关的临床和实验室参数之间的相关性。所有多发性骨髓瘤患者和所有对照中均可检测到IL-10。与健康人相比，骨髓瘤患者的IL-10浓度显著升高（平均值分别为7.09和2.1 pg/ml）（p = 0.008）。根据Salmon和Durie分类法估计，IL-10水平与疾病晚期呈正相关（I期与III期相比 - p = 0.03）。轻链病、高钙血症患者的IL-10值较高，且与C反应蛋白（CRP）浓度升高相关。121例患者中的117例以及所有对照中均可检测到IL-6。与对照组相比，MM患者中IL-6浓度有统计学意义的升高（平均值分别为16.06和4.49 pg/ml）（p = 0.01）。我们发现MM患者血清中IL-10与IL-6水平呈正相关。以Spearman等级和系数表示的这种关系（rho = 0.249，p = 0.006）具有显著性。121例MM患者中的26例以及28名健康受试者中的3例可检测到IL-11，平均浓度分别为1.2和0.6 pg/ml（p > 0.05）。121例患者中的51例以及28名对照中的仅4例可检测到可测量水平的OSM（平均值分别为3.84和0.1 pg/ml，p = 0.002）。MM患者中IL-10与IL-11水平之间的相关性不显著，但IL-10与OSM浓度之间存在强统计学相关性（rho = 0.327，p = 0.0002）。所有患者和所有对照中均可测量sIL-6R的血清浓度（平均值分别为66.00和39.57 ng/ml），但两组之间的差异不显著。我们发现IL-10与sIL-6R水平之间存在显著正相关（rho = 0.233，p = 0.01）。总之，我们指出MM患者血清中IL-10、IL-6、OSM和sIL-6R浓度可能是评估疾病活动的有用标志物。