Robinson W F, Connole M D, King T J, Pitt J I, Moss S M
School of Veterinary Science, University of Queensland, St Lucia.
Aust Vet J. 2000 Sep;78(9):600-2. doi: 10.1111/j.1751-0813.2000.tb11927.x.
A 4-year-old, entire female, German Shepherd Dog was referred with a 3-month history of right foreleg lameness that partially responded to nonsteroidal anti-inflammatory and antimicrobial therapy. The bitch lost weight, was polydipsic and had reduced exercise tolerance. On referral, the animal was in poor condition, pyrexic and exhibited moderate pain on full extension of the right shoulder. Blood, urine and joint fluid were obtained and radiographs were taken of the right shoulder and chest. The bitch was lymphopaenic, hyperfibrinogenaemic, hyperglobulinaemic, mildly azotaemic, mildly proteinuric and isosthenuric. Branching fungal hyphae were present in the urine. On radiography, the thorax contained a large ventral mediastinal mass and the humeral head had extensive areas of radiolucency. An aspirate from the right humeroscapular joint exhibited branched fungal hyphae and numerous neutrophils and macrophages. A diagnosis of disseminated mycosis was made and euthanasia was performed. At necropsy, numerous caseating granulomas were present, especially in the kidneys, adrenal glands, heart and lymph nodes. Extensive osteomyelitis involved the head of the right humerus, the sternebrae and the fifth intervertebral disc. Fungal hyphae were detected in sections of granulomas in all affected organs and a diagnosis of disseminated fungal granulomatosis was made. Aspergillus deflectus was readily isolated from affected lymph nodes, but confirming its identity as A deflectus using standard procedures proved difficult. The identity of the fungus was finally confirmed by sequencing part of the 185 rRNA of the isolate. This is the first report in Australia of a disseminated mycosis caused by A deflectus. Previously, the involvement of A deflectus as a cause of disseminated mycosis was limited to 5 cases from the West Coast of the USA, four of which occurred in German Shepherd Dogs.
一只4岁的未绝育雌性德国牧羊犬因右前肢跛行3个月前来就诊。非甾体抗炎药和抗菌治疗对此有部分疗效。该母犬体重减轻，多饮，运动耐受力下降。就诊时，动物状况不佳，发热，右肩部完全伸展时表现出中度疼痛。采集了血液、尿液和关节液，并对右肩部和胸部进行了X光检查。该母犬淋巴细胞减少、纤维蛋白原血症、球蛋白血症、轻度氮血症、轻度蛋白尿和等渗尿。尿液中存在分支真菌菌丝。X光检查显示，胸部有一个大的腹侧纵隔肿块，肱骨头有广泛的透光区。右肩肱关节穿刺液显示有分支真菌菌丝以及大量中性粒细胞和巨噬细胞。诊断为播散性霉菌病并实施了安乐死。尸检时发现大量干酪样肉芽肿，尤其是在肾脏、肾上腺、心脏和淋巴结中。广泛的骨髓炎累及右肱骨头、胸骨和第五个椎间盘。在所有受影响器官的肉芽肿切片中均检测到真菌菌丝，诊断为播散性真菌肉芽肿病。从受影响的淋巴结中很容易分离出偏侧曲霉菌，但使用标准程序确认其为偏侧曲霉菌却很困难。最终通过对分离株的185 rRNA的部分进行测序确认了真菌的身份。这是澳大利亚首例由偏侧曲霉菌引起的播散性霉菌病报告。此前，偏侧曲霉菌作为播散性霉菌病病因的病例仅限于美国西海岸的5例，其中4例发生在德国牧羊犬身上。
