Afonso A, Macedo P M, Ellis A E, Silva M T
Institute for Molecular and Cell Biology, University of Porto, Portugal.
Dis Aquat Organ. 2000 Aug 31;42(2):101-10. doi: 10.3354/dao042101.
The ultrastructural image of glycogen granules in the cytoplasm of rainbow trout phagocytes in sections stained by the conventional lead or uranyl-lead stains is highly dependent on fixation conditions, the granules being visible only when adequate fixation protocols are used. Morphometry of samples processed for the detection of peroxidase or esterase activities (to specifically label neutrophils and macrophages, respectively), and simultaneously stained for the specific detection of glycogen, showed that inflammatory peritoneal neutrophils were richer in glycogen granules than resting neutrophils. This increase in glycogen content occurs after the migration from the haematopoietic tissues and peripheral blood to the inflamed foci. Glycogen granules could not be found in resting peritoneal macrophages but were found in inflammatory macrophages. The macrophage granules occurred in smaller amounts than in neutrophils, and consisted of granules identical to those of neutrophils together with significantly smaller granules. No evidence for the utilization of glycogen by neutrophils phagocytosing bacteria within the peritoneal cavity was found.
在用传统铅染或铀铅染染色的切片中，虹鳟吞噬细胞胞质中糖原颗粒的超微结构图像高度依赖于固定条件，只有采用适当的固定方案时颗粒才可见。对用于检测过氧化物酶或酯酶活性（分别特异性标记中性粒细胞和巨噬细胞）并同时进行糖原特异性染色的样本进行形态测量，结果显示，炎症性腹膜中性粒细胞比静息中性粒细胞富含更多的糖原颗粒。糖原含量的这种增加发生在从造血组织和外周血迁移到炎症病灶之后。在静息腹膜巨噬细胞中未发现糖原颗粒，但在炎症巨噬细胞中发现了糖原颗粒。巨噬细胞中的颗粒数量比中性粒细胞中的少，由与中性粒细胞相同的颗粒以及明显更小的颗粒组成。未发现腹膜腔内吞噬细菌的中性粒细胞利用糖原的证据。
