Kim Do-Hyung, Austin Brian
School of Life Sciences, John Muir Building, Heriot-Watt University, Riccarton, Edinburgh EH14 4AS, Scotland, UK.
Fish Shellfish Immunol. 2006 Nov;21(5):513-24. doi: 10.1016/j.fsi.2006.02.007. Epub 2006 Mar 2.
Carnobacterium maltaromaticum B26 and Carnobacterium divergens B33, which were isolated from the intestine of healthy rainbow trout (Oncorhynchus mykiss, Walbaum), were selected as being potentially useful as probiotics with effectiveness against Aeromonas salmonicida and Yersinia ruckeri. Thus, rainbow trout administered with feed supplemented with B26 or B33 dosed at >10(7) cells g(-1) feed conferred protection against challenge with virulent cultures of the pathogens. Moreover, both cultures persisted in the gut for up to 3 weeks after administration. The cultures enhanced the cellular and humoral immune responses. Specifically, fish fed with B26 demonstrated significantly increased phagocytic activity of the head kidney macrophages, whereas the use of B33 led to significant increases in respiratory burst and serum lysozyme activity. Also, the gut mucosal lysozyme activity for fish fed with both cultures was statistically higher than the controls.
从健康虹鳟（Oncorhynchus mykiss，Walbaum）肠道中分离出的麦芽香肉杆菌B26和分歧肉杆菌B33，被选为可能具有益生菌作用且对杀鲑气单胞菌和鲁氏耶尔森菌有效的菌株。因此，投喂添加了剂量大于10⁷ 个细胞/g饲料的B26或B33的虹鳟，对病原体的强毒培养物攻击具有保护作用。此外，两种培养物在投喂后在肠道中持续存在长达3周。这些培养物增强了细胞免疫和体液免疫反应。具体而言，投喂B26的鱼，其头肾巨噬细胞的吞噬活性显著增加，而使用B33则导致呼吸爆发和血清溶菌酶活性显著增加。而且，投喂两种培养物的鱼的肠道黏膜溶菌酶活性在统计学上高于对照组。
