Jakubowski W, Bartosz G
Department of Molecular Biophysics, University of Lódz, Banacha 12/16, Lódz, 90-237, Poland.
Cell Biol Int. 2000;24(10):757-60. doi: 10.1006/cbir.2000.0556.
Intracellular 2,7-dichlorofluorescin (H(2)DCF) oxidation is often used to measure the production of reactive oxygen species (ROS) within cells. The rate of H(2)DCF oxidation depends on the concentration of glutathione, which is an alternative target for ROS. Our results suggest that increased rate of H(2)DCF oxidation be interpreted as an indication of general oxidative stress due to a variety of reasons, including depletion of antioxidants, rather than as a specific proof of augmented ROS formation.
细胞内2,7 - 二氯荧光素（H₂DCF）氧化常用于测量细胞内活性氧（ROS）的产生。H₂DCF氧化速率取决于谷胱甘肽的浓度，而谷胱甘肽是ROS的另一个作用靶点。我们的结果表明，H₂DCF氧化速率增加应被解释为由于多种原因（包括抗氧化剂耗竭）导致的一般氧化应激的指标，而不是ROS生成增加的具体证据。
