Immunofluorescent staining of gastric mucosal glycoprotein in gastric carcinoma of dogs and rats induced by N-methyl-N'-nitro-N-nitrosoguanidine.

Immunohistological studies, using the fluorescein isothiocyanate-labeled antibody against gastric mucosal glycoprotein, were made during the development of gastric cancer, induced in dogs and rats by oral administration of N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). In an early stage, the regenerative glands were lined by fluorescent mucus cells. Carcinoma cells of orderly glandular structure, produced in dogs, were devoid of fluorescence. Carcinoma cells of less differentiation, produced in rats during further advanced stage, were well fluorescent. The immunofluorescent profiles of such experimentally induced gastric carcinoma were found to be the same as those of human gastric adenocarcinoma.