Köhling R, Höhling J M, Straub H, Kuhlmann D, Kuhnt U, Tuxhorn I, Ebner A, Wolf P, Pannek H W, Gorji A, Speckmann E J
Institut für Physiologie, Westfälische Wilhelms-Universität, 48149 Münster, Germany.
J Neurophysiol. 2000 Oct;84(4):2161-5. doi: 10.1152/jn.2000.84.4.2161.
Functional changes in neuronal circuitry reflected in spontaneously occurring synchronous sharp field potentials (SSFP) have been reported to occur in human brain suffering from chronic epileptogenicity but not in primary nonepileptic tissue from peritumoral resectates. Voltage sensitive dyes and fast imaging were used to visualize spontaneously occurring rhythmic depolarizations correlated to SSFP in chronically epileptic human neocortical slices obtained during epilepsy surgery. Localized and spatially inhomogeneous neuronal depolarizations were found to underlie spontaneous SSFP, which remained unchanged and spatially restricted to foci <750 micrometer diam even under epileptogenic (low-Mg(2+)) conditions. In cases where ictaform paroxysmal activity occurred in low-Mg(2+) medium, neuronal depolarizations were wide-spread but still spatially inhomogeneous, and the events were preferentially initiated at distinct foci. The findings suggest that small neuronal networks are able to establish and maintain synchronous rhythmic and epileptiform activity.
据报道，在患有慢性癫痫源性的人脑中会出现反映在自发同步锐波场电位（SSFP）中的神经回路功能变化，但在肿瘤周围切除组织的原发性非癫痫组织中则不会出现。电压敏感染料和快速成像技术被用于可视化在癫痫手术期间获得的慢性癫痫性人类新皮层切片中与SSFP相关的自发节律性去极化。研究发现，局部且空间上不均匀的神经元去极化是自发SSFP的基础，即使在癫痫源性（低镁离子）条件下，这种去极化仍保持不变且在空间上局限于直径<750微米的病灶。在低镁离子培养基中出现癫痫样阵发性活动的情况下，神经元去极化广泛存在但在空间上仍不均匀，并且这些事件优先在不同的病灶处起始。这些发现表明，小神经元网络能够建立并维持同步的节律性和癫痫样活动。
