Hook E B, Hatcher N H, Calka O J
Humangenetik. 1975 Sep 20;30(3):251-7. doi: 10.1007/BF00279190.
Six adjacent metaphases, each with the same cytogenetic aberration of a group D chromosome, most probably a No. 14, were observed in a field of a slide from a 96-hour culture of lymphocytes from an individual with ataxia-telangiectasia (AT). None of the 304 other metaphases examined from this or other simultaneous cultures of this individual showed such an aberration. It seems most likely that an "in situ" marked clone has been observed and this supports interpretation of consistent cytogenetic abnormalities in those with AT as having clonal origin. The method of slide preparation employed which involves placing, rather than dropping, the cell suspension on the slide may facilitate detection of "in situ" clones.
在一张来自共济失调毛细血管扩张症（AT）患者淋巴细胞96小时培养物的玻片视野中，观察到六个相邻的中期分裂相，每个都具有D组染色体相同的细胞遗传学畸变，很可能是14号染色体。从该个体的此次培养物或其他同时期培养物中检查的304个其他中期分裂相中，均未显示出这种畸变。似乎最有可能的是观察到了一个“原位”标记克隆，这支持了将AT患者中一致的细胞遗传学异常解释为具有克隆起源的观点。所采用的玻片制备方法，即将细胞悬液放置而非滴加在玻片上，可能有助于检测“原位”克隆。
