Choi H, Kim S, Son J, Hong S, Lee H, Lee H
Samyang Genex Biotech Research Institute, 305-348, Taejon, South Korea
Enzyme Microb Technol. 2000 Nov 1;27(8):593-598. doi: 10.1016/s0141-0229(00)00255-6.
Effect of temperature shift during culture period on cell growth and paclitaxel was investigated to optimize paclitaxel production in suspension culture of Taxus chinensis. Cell growth showed the optimum at 24 degrees C while paclitaxel synthesis showed the maximum at 29 degrees C. To minimize the inhibitory effect of higher temperature on cell growth, temperature was shifted after a certain period of culture time at 24 degrees C. Paclitaxel synthesis in plant cell culture increased dramatically during day 14 to day 21 regardless of treatment, reaching the maximum production of 137.5 mg paclitaxel/L. When the temperature was maintained at 29 degrees C after day 21, the specific productivity of paclitaxel was sustained for prolonged period of 42 days. The possible relationship between temperature and paclitaxel synthetic pathway was also suggested.
研究了培养期间温度变化对中国红豆杉悬浮培养中细胞生长和紫杉醇的影响，以优化紫杉醇的生产。细胞生长在24℃时表现最佳，而紫杉醇合成在29℃时达到最大值。为了使较高温度对细胞生长的抑制作用最小化，在24℃培养一段时间后改变温度。无论处理如何，植物细胞培养中的紫杉醇合成在第14天到第21天期间急剧增加，达到最大产量137.5mg紫杉醇/L。在第21天后将温度维持在29℃时，紫杉醇的比生产率持续了42天的较长时间。还提出了温度与紫杉醇合成途径之间可能的关系。
