Centre for Foundation Studies in Science, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia ; Centre of Biotechnology for Agriculture Research (CEBAR), Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
Centre of Biotechnology for Agriculture Research (CEBAR), Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia ; Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
Evid Based Complement Alternat Med. 2015;2015:757514. doi: 10.1155/2015/757514. Epub 2015 Feb 12.
Zingiber zerumbet Smith is an important herb that contains bioactive phytomedicinal compound, zerumbone. To enhance cell growth and production of this useful compound, we investigated the growth conditions of cell suspension culture. Embryogenic callus generated from shoot bud was used to initiate cell suspension culture. The highest specific growth rate of cells was recorded when it was cultured in liquid Murashige and Skoog basal medium containing 3% sucrose with pH 5.7 and incubated under continuous shaking condition of 70 rpm for 16 h light and 8 h dark cycle at 24°C. Our results also revealed that the type of carbohydrate substrate, light regime, agitation speed, and incubation temperature could affect the production of zerumbone. Although the zerumbone produced in this study was not abundant compared to rhizome of Z. zerumbet, the possibility of producing zerumbone during early stage could serve as a model for subsequent improvement.
盾叶薯蓣是一种重要的草药，含有生物活性的植物药化合物，莪术醇。为了提高细胞生长和这种有用化合物的产量，我们研究了细胞悬浮培养的生长条件。从芽点生成的胚性愈伤组织被用来启动细胞悬浮培养。当在含有 3%蔗糖的液体 Murashige 和 Skoog 基本培养基中培养，pH 值为 5.7，并在 24°C 下以 70rpm 的连续搅拌条件下，16 小时光照和 8 小时黑暗周期中培养时，细胞的比生长速率最高。我们的结果还表明，碳水化合物底物的类型、光照制度、搅拌速度和培养温度都会影响莪术醇的产量。虽然与盾叶薯蓣的根茎相比，本研究中产生的莪术醇并不丰富，但在早期产生莪术醇的可能性可以作为后续改进的模型。
