Hashimoto H, Yabe T, Hirata T, Shimizu T, Bae Y, Yamanaka Y, Hirano T, Hibi M
Division of Molecular Oncology (C7), Biomedical Research Center, Osaka University Graduate School of Medicine, 2-2 Yamada-oka, Suita, 565-0871, Osaka, Japan.
Mech Dev. 2000 Oct;97(1-2):191-5. doi: 10.1016/s0925-4773(00)00418-4.
Anterior-posterior (A-P) patterning in the neuroectoderm is established during gastrulation in zebrafish and amphibians. We isolated a novel zinc-finger gene fez-like (fezl) from zebrafish, which displays sequence similarities to Xenopus Fez. The fezl transcripts were detected in the anterior edge of neuroectoderm, the prospective dorsal forebrain, from the late gastrula (80% epiboly stage) to the mid-segmentation period. fezl was also expressed in the ventral forebrain overlying the prechordal plate at these stages. The expression of fezl was enhanced in embryos expressing the Wnt inhibitor Dkk1 and reduced in embryos expressing Wnt8b. The expression in the ventral forebrain was eliminated in the one-eyed pinhead mutant and the antivin RNA-injected embryos, which lack the prechordal plate. Radiation hybrid mapping revealed that the fezl gene is localized to linkage group 11.
在斑马鱼和两栖动物的原肠胚形成过程中，神经外胚层的前后（A-P）模式得以确立。我们从斑马鱼中分离出一个新的锌指基因fez样基因（fezl），它与非洲爪蟾的Fez具有序列相似性。fezl转录本在神经外胚层的前缘、预期的背侧前脑被检测到，从原肠胚晚期（80%外包期）到中分割期。在这些阶段，fezl也在前索板上方的腹侧前脑中表达。在表达Wnt抑制剂Dkk1的胚胎中，fezl的表达增强，而在表达Wnt8b的胚胎中，fezl的表达降低。在缺少前索板的独眼小头突变体和注射抗维甲酸RNA的胚胎中，腹侧前脑的表达消失。辐射杂种图谱分析表明，fezl基因定位于连锁群11。
