• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

FEZF1-AS1/miR-107/ZNF312B 轴促进胰腺导管腺癌的进展和瓦博格效应。

FEZF1-AS1/miR-107/ZNF312B axis facilitates progression and Warburg effect in pancreatic ductal adenocarcinoma.

机构信息

Department of Hepatopancreatobiliary Surgery, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, Guangdong Province, China.

Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, Guangdong Province, China.

出版信息

Cell Death Dis. 2018 Jan 18;9(2):34. doi: 10.1038/s41419-017-0052-1.

DOI:10.1038/s41419-017-0052-1
PMID:29348628
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5833349/
Abstract

Long non-coding RNAs (lncRNAs) play a pivotal role in pathological processes. However, little information has been published regarding the underlying functions and mechanisms of lncRNAs in pancreatic ductal adenocarcinoma (PDAC). A novel lncRNA FEZF1-AS1 and its sense-cognate gene ZNF312B were found to be highly expressed in human PDAC tissues and cell lines, which is associated with disease progression and predicts clinical outcome in PDAC patients. Of note, bioinformatics analysis, luciferase assays and RNA immunoprecipitation assays indicated that FEZF1-AS1 may act as an endogenous sponge by competing for miR-107, thereby modulating the derepression of ZNF312B. Downregulation of FEZF1-AS1 or ZNF312B significantly inhibited proliferation, colony formation, migration, and invasion of PDAC cells in vitro, whereas the miR-107 inhibitor abrogated the effect of dow-regulation of FEZF1-AS1 or ZNF312B in reducing oncogenic capacities of PDAC cells. In addition, FEZF1-AS1/miR-107/ZNF312B axis-induced promotion of PDAC cells proliferation appeared to be mediated by modulation of the apoptosis and the G1-S checkpoint. Furthermore, downregulation of FEZF1-AS1 repressed tumor growth in mouse xenograft models. In particular, our results highlight the contribution of FEZF1-AS1/miR-107/ZNF312B axis to Warburg effect maintenance of PDAC cells. Collectively, our findings demonstrate that the FEZF1-AS1/miR-107/ZNF312B axis regulatory network might provide a potential new therapeutic strategy for PDAC.

摘要

长链非编码 RNA（lncRNA）在病理过程中发挥着关键作用。然而，关于 lncRNA 在胰腺导管腺癌（PDAC）中的潜在功能和机制，目前发表的信息很少。我们发现一种新型 lncRNA FEZF1-AS1 及其有意义的同源基因 ZNF312B 在人 PDAC 组织和细胞系中高度表达，与疾病进展相关，并可预测 PDAC 患者的临床结局。值得注意的是，生物信息学分析、荧光素酶报告基因实验和 RNA 免疫沉淀实验表明，FEZF1-AS1 可能作为内源性海绵通过与 miR-107 竞争，从而调节 ZNF312B 的去抑制。下调 FEZF1-AS1 或 ZNF312B 显著抑制 PDAC 细胞在体外的增殖、集落形成、迁移和侵袭，而 miR-107 抑制剂消除了下调 FEZF1-AS1 或 ZNF312B 降低 PDAC 细胞致癌能力的作用。此外，FEZF1-AS1/miR-107/ZNF312B 轴诱导的 PDAC 细胞增殖促进似乎是通过调节细胞凋亡和 G1-S 检验点来介导的。此外，下调 FEZF1-AS1 抑制了小鼠异种移植模型中的肿瘤生长。特别是，我们的研究结果强调了 FEZF1-AS1/miR-107/ZNF312B 轴对 PDAC 细胞 Warburg 效应维持的贡献。总之，我们的研究结果表明，FEZF1-AS1/miR-107/ZNF312B 轴调控网络可能为 PDAC 提供一种潜在的新治疗策略。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b1c/5833349/093b5c0480a8/41419_2017_52_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b1c/5833349/2ab1cd7c8e63/41419_2017_52_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b1c/5833349/cb0843e84fa5/41419_2017_52_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b1c/5833349/bf288e535fe0/41419_2017_52_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b1c/5833349/ff9813be2fe8/41419_2017_52_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b1c/5833349/f8757790f5ca/41419_2017_52_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b1c/5833349/ad7d3605f4fe/41419_2017_52_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b1c/5833349/316fa49a3013/41419_2017_52_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b1c/5833349/093b5c0480a8/41419_2017_52_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b1c/5833349/2ab1cd7c8e63/41419_2017_52_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b1c/5833349/cb0843e84fa5/41419_2017_52_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b1c/5833349/bf288e535fe0/41419_2017_52_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b1c/5833349/ff9813be2fe8/41419_2017_52_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b1c/5833349/f8757790f5ca/41419_2017_52_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b1c/5833349/ad7d3605f4fe/41419_2017_52_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b1c/5833349/316fa49a3013/41419_2017_52_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b1c/5833349/093b5c0480a8/41419_2017_52_Fig8_HTML.jpg

相似文献

1
FEZF1-AS1/miR-107/ZNF312B axis facilitates progression and Warburg effect in pancreatic ductal adenocarcinoma.FEZF1-AS1/miR-107/ZNF312B 轴促进胰腺导管腺癌的进展和瓦博格效应。
Cell Death Dis. 2018 Jan 18;9(2):34. doi: 10.1038/s41419-017-0052-1.
2
lncRNA Promotes Pancreatic Ductal Adenocarcinoma Growth and Leads to a Poor Clinical Outcome via Sponging miR-484 and Interacting with YAP.长链非编码 RNA 通过海绵吸附 miR-484 并与 YAP 相互作用促进胰腺导管腺癌生长并导致不良临床结局。
Clin Cancer Res. 2020 Apr 1;26(7):1736-1748. doi: 10.1158/1078-0432.CCR-19-0674. Epub 2019 Dec 12.
3
Long non-coding RNA FEZF1-AS1 promotes cell growth in multiple myeloma via miR-610/Akt3 axis.长非编码 RNA FEZF1-AS1 通过 miR-610/Akt3 轴促进多发性骨髓瘤细胞生长。
Biomed Pharmacother. 2018 Jul;103:1727-1732. doi: 10.1016/j.biopha.2018.04.094.
4
Long non-coding RNA CERS6-AS1 facilitates the oncogenicity of pancreatic ductal adenocarcinoma by regulating the microRNA-15a-5p/FGFR1 axis.长链非编码 RNA CERS6-AS1 通过调控 microRNA-15a-5p/FGFR1 轴促进胰腺导管腺癌的致癌性。
Aging (Albany NY). 2021 Feb 13;13(4):6041-6054. doi: 10.18632/aging.202540.
5
Long non-coding RNA FEZF1-AS1 induced progression of ovarian cancer via regulating miR-130a-5p/SOX4 axis.长链非编码 RNA FEZF1-AS1 通过调控 miR-130a-5p/SOX4 轴促进卵巢癌细胞的进展。
J Cell Mol Med. 2020 Apr;24(7):4275-4285. doi: 10.1111/jcmm.15088. Epub 2020 Mar 5.
6
Long Noncoding RNA PSMA3 Antisense RNA 1 Promotes Cell Proliferation, Migration, and Invasion in Pancreatic Ductal Adenocarcinoma Via Targeting MicroRNA-154-5p to Positively Modulate Karyopherin Subunit Alpha 4.长链非编码RNA PSMA3反义RNA 1通过靶向微小RNA-154-5p正向调节核转运蛋白亚基α4，促进胰腺导管腺癌的细胞增殖、迁移和侵袭。
Pancreas. 2022 Sep 1;51(8):1037-1046. doi: 10.1097/MPA.0000000000002136.
7
Linc00511 acts as a competing endogenous RNA to regulate VEGFA expression through sponging hsa-miR-29b-3p in pancreatic ductal adenocarcinoma.Linc00511 通过海绵吸附 hsa-miR-29b-3p 来调节 VEGFA 表达，充当胰腺导管腺癌的竞争性内源性 RNA。
J Cell Mol Med. 2018 Jan;22(1):655-667. doi: 10.1111/jcmm.13351. Epub 2017 Oct 5.
8
High expression of AFAP1-AS1 is associated with poor survival and short-term recurrence in pancreatic ductal adenocarcinoma.AFAP1-AS1的高表达与胰腺导管腺癌的不良生存和短期复发相关。
J Transl Med. 2015 Apr 30;13:137. doi: 10.1186/s12967-015-0490-4.
9
Mechanism of lncRNA FEZF1-AS1 in promoting the occurrence and development of oral squamous cell carcinoma through targeting miR-196a.lncRNA FEZF1-AS1 通过靶向 miR-196a 促进口腔鳞状细胞癌发生发展的机制。
Eur Rev Med Pharmacol Sci. 2019 Aug;23(15):6505-6515. doi: 10.26355/eurrev_201908_18534.
10
LncRNA FEZF1-AS1 enhances epithelial-mesenchymal transition (EMT) through suppressing E-cadherin and regulating WNT pathway in non-small cell lung cancer (NSCLC).长链非编码 RNA FEZF1-AS1 通过抑制 E-钙黏蛋白和调节非小细胞肺癌（NSCLC）中的 WNT 通路来增强上皮-间充质转化（EMT）。
Biomed Pharmacother. 2017 Nov;95:331-338. doi: 10.1016/j.biopha.2017.08.057. Epub 2017 Sep 12.

引用本文的文献

1
FEZF1-AS1 drives autophagy-mediated progression of colon cancer and reduces chemosensitivity through inhabiting the PI3K/AKT/mTOR signaling pathway.FEZF1反义RNA1通过抑制PI3K/AKT/mTOR信号通路驱动自噬介导的结肠癌进展并降低化疗敏感性。
Front Genet. 2025 Jul 24;16:1514205. doi: 10.3389/fgene.2025.1514205. eCollection 2025.
2
LncRNAs in hypoxic microenvironment; insight in their impact in cancer biology.缺氧微环境中的长链非编码RNA；对其在癌症生物学中影响的见解
Funct Integr Genomics. 2025 Jul 2;25(1):142. doi: 10.1007/s10142-025-01635-9.
3
circRNA-PTPN4 mediated regulation of FOXO3 and ZO-1 expression: implications for blood-brain barrier integrity and cognitive function in uremic encephalopathy.

本文引用的文献

1
Long noncoding RNA CRNDE stabilized by hnRNPUL2 accelerates cell proliferation and migration in colorectal carcinoma via activating Ras/MAPK signaling pathways.由hnRNPUL2稳定的长链非编码RNA CRNDE通过激活Ras/MAPK信号通路加速结直肠癌细胞的增殖和迁移。
Cell Death Dis. 2017 Jun 8;8(6):e2862. doi: 10.1038/cddis.2017.258.
2
Cancer Statistics, 2017.《2017 年癌症统计》
CA Cancer J Clin. 2017 Jan;67(1):7-30. doi: 10.3322/caac.21387. Epub 2017 Jan 5.
3
ARF6, induced by mutant Kras, promotes proliferation and Warburg effect in pancreatic cancer.
环状 RNA-PTPN4 调控 FOXO3 和 ZO-1 的表达：对尿毒症脑病血脑屏障完整性和认知功能的影响。
Cell Biol Toxicol. 2024 Apr 17;40(1):22. doi: 10.1007/s10565-024-09865-6.
4
MicroRNA-Mediated Regulation of Histone-Modifying Enzymes in Cancer: Mechanisms and Therapeutic Implications.miRNA 介导的癌症中组蛋白修饰酶的调节：机制和治疗意义。
Biomolecules. 2023 Oct 28;13(11):1590. doi: 10.3390/biom13111590.
5
Noncoding RNAs in apoptosis: identification and function.细胞凋亡中的非编码RNA：鉴定与功能
Turk J Biol. 2021 Nov 14;46(1):1-40. doi: 10.3906/biy-2109-35. eCollection 2022.
6
The LncRNA FEZF1-AS1 promotes tumor proliferation in colon cancer by regulating the mitochondrial protein PCK2.长链非编码 RNA FEZF1-AS1 通过调节线粒体蛋白 PCK2 促进结肠癌肿瘤增殖。
Oncol Res. 2022 Aug 1;29(3):201-215. doi: 10.32604/or.2022.03553. eCollection 2021.
7
Lncrna FEZf1-as1 negatively regulates ETNK1 to promote malignant progression of renal cell carcinoma.长链非编码RNA FEZf1-as1通过负向调控ETNK1促进肾细胞癌的恶性进展。
J Med Biochem. 2023 Mar 15;42(2):232-238. doi: 10.5937/jomb0-39710.
8
Tumor-suppressive miR-29c binds to MAPK1 inhibiting the ERK/MAPK pathway in pancreatic cancer.抑癌 miR-29c 与 MAPK1 结合，抑制胰腺癌中的 ERK/MAPK 通路。
Clin Transl Oncol. 2023 Mar;25(3):803-816. doi: 10.1007/s12094-022-02991-9. Epub 2022 Dec 12.
9
Radio-lncRNAs: Biological Function and Potential Use as Biomarkers for Personalized Oncology.放射性长链非编码RNA：生物学功能及作为个性化肿瘤学生物标志物的潜在用途
J Pers Med. 2022 Sep 29;12(10):1605. doi: 10.3390/jpm12101605.
10
Nerve Growth Factor (NGF) Encourages the Neuroinvasive Potential of Pancreatic Cancer Cells by Activating the Warburg Effect and Promoting Tumor Derived Exosomal miRNA-21 Expression.神经生长因子（NGF）通过激活瓦博格效应和促进肿瘤衍生外泌体 miRNA-21 表达来增强胰腺癌细胞的神经侵袭潜力。
Oxid Med Cell Longev. 2022 Oct 15;2022:8445093. doi: 10.1155/2022/8445093. eCollection 2022.
由突变型Kras诱导产生的ARF6可促进胰腺癌的增殖和瓦伯格效应。
Cancer Lett. 2017 Mar 1;388:303-311. doi: 10.1016/j.canlet.2016.12.014. Epub 2016 Dec 23.
4
Energy sources identify metabolic phenotypes in pancreatic cancer.能量来源可识别胰腺癌的代谢表型。
Acta Biochim Biophys Sin (Shanghai). 2016 Nov;48(11):969-979. doi: 10.1093/abbs/gmw097. Epub 2016 Sep 20.
5
Long non-coding RNA FEZF1-AS1 facilitates cell proliferation and migration in colorectal carcinoma.长链非编码RNA FEZF1-AS1促进结直肠癌细胞的增殖和迁移。
Oncotarget. 2016 Mar 8;7(10):11271-83. doi: 10.18632/oncotarget.7168.
6
Pancreatic cancer.胰腺癌。
Lancet. 2016 Jul 2;388(10039):73-85. doi: 10.1016/S0140-6736(16)00141-0. Epub 2016 Jan 30.
7
The long non-coding RNA HOTTIP promotes progression and gemcitabine resistance by regulating HOXA13 in pancreatic cancer.长链非编码RNA HOTTIP通过调控HOXA13促进胰腺癌进展及吉西他滨耐药。
J Transl Med. 2015 Mar 12;13:84. doi: 10.1186/s12967-015-0442-z.
8
Long noncoding RNA profiles of adrenocortical cancer can be used to predict recurrence.肾上腺皮质癌的长链非编码RNA图谱可用于预测复发。
Endocr Relat Cancer. 2015 Feb;22(1):99-109. doi: 10.1530/ERC-14-0457.
9
Lnc RNA HOTAIR functions as a competing endogenous RNA to regulate HER2 expression by sponging miR-331-3p in gastric cancer.长链非编码RNA HOTAIR作为一种竞争性内源性RNA，通过吸附胃癌中的miR-331-3p来调节HER2表达。
Mol Cancer. 2014 Apr 28;13:92. doi: 10.1186/1476-4598-13-92.
10
RNAi factors are present and active in human cell nuclei.RNAi 因子存在于并活跃于人类细胞核中。
Cell Rep. 2014 Jan 16;6(1):211-21. doi: 10.1016/j.celrep.2013.12.013. Epub 2014 Jan 2.