一种在非洲爪蟾和小鼠胚胎前脑中转录的新型锌指基因Fez的克隆与表达。
Cloning and expression of a novel zinc finger gene, Fez, transcribed in the forebrain of Xenopus and mouse embryos.
作者信息
Matsuo-Takasaki M, Lim J H, Beanan M J, Sato S M, Sargent T D
机构信息
Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA.
出版信息
Mech Dev. 2000 May;93(1-2):201-4. doi: 10.1016/s0925-4773(00)00264-1.
We have identified and cloned a novel zinc finger gene, Fez (forebrain embryonic zinc-finger), as a potential downstream determinant of anterior neural plate formation in Xenopus. Fez was isolated as one of several neural-specific genes that was induced by the neuralizing factor, noggin (Smith and Harland, 1992. Cell 70, 829-840), in uncommitted ectoderm. Fez has an open reading frame comprising 466 amino acids, and contains six C(2)H(2) type zinc finger domains, which are highly conserved among Drosophila, zebrafish, mouse, and human. In Xenopus, the expression of Fez begins at stage 12 in the rostral end of the neural plate, and by stage 45, it is localized to several telencephalic regions, including the olfactory bulbs, nervus terminalis, and ventricular zone. The mouse homologue of Fez is similarly expressed in the mouse forebrain by embryonic day 11.
我们已经鉴定并克隆了一个新的锌指基因Fez（前脑胚胎锌指基因），它是非洲爪蟾前神经板形成的一个潜在下游决定因素。Fez是作为几个神经特异性基因之一被分离出来的，这些基因在未分化的外胚层中由神经化因子头蛋白（Smith和Harland，1992年。《细胞》70卷，829 - 840页）诱导产生。Fez有一个包含466个氨基酸的开放阅读框，并含有六个C(2)H(2)型锌指结构域，这些结构域在果蝇、斑马鱼、小鼠和人类中高度保守。在非洲爪蟾中，Fez的表达始于神经板前端的第12期，到第45期时，它定位于几个端脑区域，包括嗅球、终神经和脑室区。Fez的小鼠同源物在胚胎第11天时在小鼠前脑中也有类似的表达。
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