Tulinska J, Dusinska M, Jahnova E, Liskova A, Kuricova M, Vodicka P, Vodickova L, Sulcova M, Fuortes L
Institute of Preventive and Clinical Medicine, Department of Immunotoxicology, Limbova 14, 833 01 Bratislava, Slovak Republic.
Am J Ind Med. 2000 Nov;38(5):576-83. doi: 10.1002/1097-0274(200011)38:5<576::aid-ajim10>3.0.co;2-f.
Styrene is a widely used industrial chemical. Immune and hematological parameters were examined in 29 hand laminators and sprayers exposed to styrene for an average of 14 years and in 19 in-factory unexposed controls. The workers performed hand lamination procedures in a production area with an average area airborne styrene level of 139.5 mg/m(3). Mean concentration of styrene in the blood of exposed workers was 945.7 microg/L and the mean styrene in exhaled air was 38.8 microg/L.
Parameters of internal and external exposure, immune function assays, immunoglobulins, acute phase reactants and hematology were evaluated in exposed and non-exposed populations.
Using multifactorial analysis of variance we found a significant decrease in proliferation of lymphocytes stimulated by Concanavalin A but not by pokeweed mitogen (PWM) in workers occupationally exposed to styrene. Proliferative response to PWM was significantly correlated with the levels of styrene in blood. Phagocytic activity of monocytes, levels of IgG, IgA, IgM, IgE and alpha-2-macroglobulin in serum were indistinguishable in the two groups. The population exposed to styrene had increased levels of C4-component of complement. Levels of C3-component of complement were positively correlated with duration of exposure. A significant elevation in the percentage and number of monocytes and a significantly decreased number of lymphocytes were seen in exposed workers. Styrene concentrations in both blood and exhaled air were associated with decreased percentage of large granular lymphocytes.
These results suggest immune alterations of cell-mediated immune response of T-lymphocytes and imbalance in leucocyte subsets in peripheral blood of workers exposed to styrene.
苯乙烯是一种广泛使用的工业化学品。对29名平均接触苯乙烯14年的手工层压机操作工和喷漆工以及19名工厂内未接触者进行了免疫和血液学参数检测。这些工人在一个生产区域进行手工层压操作，该区域空气中苯乙烯平均浓度为139.5毫克/立方米。接触工人血液中苯乙烯的平均浓度为945.7微克/升，呼出空气中苯乙烯的平均浓度为38.8微克/升。
对接触组和非接触组人群评估了内外暴露参数、免疫功能检测、免疫球蛋白、急性期反应物和血液学指标。
通过多因素方差分析，我们发现职业性接触苯乙烯的工人中，由刀豆球蛋白A刺激而非商陆有丝分裂原（PWM）刺激的淋巴细胞增殖显著降低。对PWM的增殖反应与血液中苯乙烯水平显著相关。两组中单核细胞的吞噬活性、血清中IgG、IgA、IgM、IgE和α-2-巨球蛋白水平无差异。接触苯乙烯的人群补体C4成分水平升高。补体C3成分水平与接触持续时间呈正相关。接触工人中单核细胞的百分比和数量显著升高，淋巴细胞数量显著减少。血液和呼出空气中的苯乙烯浓度与大颗粒淋巴细胞百分比降低有关。
这些结果表明，接触苯乙烯的工人T淋巴细胞的细胞介导免疫反应存在免疫改变，外周血白细胞亚群失衡。
