正常男性血清促黄体生成素（LH）和促卵泡生成素（FSH）对合成促性腺激素释放激素的反应。

Serum LH and FSH responses to synthetic gonadotropin-releasing hormone in normal men.

作者信息

Snyder P J, Reitano J F, Utiger R D

出版信息

J Clin Endocrinol Metab. 1975 Nov;41(5):938-45. doi: 10.1210/jcem-41-5-938.

Abstract

The effects of dose and of age on the serum LH and FSH responses to synthetic gonadotropin-releasing hormone (GnRH) were determined in normal men. GnRH was given as in iv bolus in doses from 2 to 500 mug to each of nine normal men, 20-39 years old. The mean (+/- SE) maximum delta LH (maximum increment above the basal concentration) in response to each dose of GnRH was 2 mug, 7.2 +/- 1.7 mIU/ml; 10 mug, 15.7 +/- 3.4 mIU/ml; 50 mug, 37.7 +/- 9.6 mIU/ml; 250 mug, 47.8 +/- 11.2 mIU/ml; and 500 mug, 49.3 +/- 11.2 mIU/ml. The mean delta FSH in response to each dose was 2 mug, 0.5 +/- 0.2 mIU/ml; 10 mug, 0.8 +/- 0.2 mIU/ml; 50 mug, 1.2 +/- 0.3 mIU/ml; 250 mug, 2.0 +/- 0.3 mIU/ml; and 500 mug, 2.4 +/- 0.4 mIU/ml. Since both the LH and FSH responses to the 250 mug dose were significantly greater than those to smaller doses, but the responses to the 500 mug dose were not still larger, 250 mug was used as the standard test done for subsequent studies. The effect of age was tested by administering a 250 mug dose of GnRH as an iv bolus to 42 carefully selected normal men, 14 in each of three age groups, 20-39, 40-59, and 60-79 years old. Dialyzable serum testosterone levels decreased with increasing age, from 21.7 +/- 4.6 to 16.0 +/- 4.9 to 14.3 +/- 3.8 ng/dl (mean +/- SD), and the basal serum LH levels increased slightly, from 7.4 +/- 3.4 to 9.6 +/- 3.1 to 10.8 +/- 3.2 mIU/ml, suggesting that a slight degree of primary Leydig cell failure occurs with increasing age. There was no corresponding increase in mean maximum delta LH in response to GnRH with increasing age, despite the elevation in mean basal LH levels. The maximum delta LH/basal LH ratio, in fact, declined from 6.8 to 5.1 to 3.3 in the three age groups. The basal serum FSH levels did not change with increasing age, but the mean delta FSH values decreased from 3.0 +/- 0.5 to 2.9 +/- 1.1 +/- 0.3 mIU/ml. These data demonstrate decreased LH and FSH responses to synthetic GnRH, as well as some primary Leydig cell failure, in the male senescence.

摘要

在正常男性中测定了剂量和年龄对血清促黄体生成素（LH）和促卵泡生成素（FSH）对合成促性腺激素释放激素（GnRH）反应的影响。对9名年龄在20至39岁的正常男性，以静脉推注的方式给予GnRH，剂量从2至500微克不等。对每个剂量的GnRH，平均（±标准误）最大LH变化量（高于基础浓度的最大增量）分别为：2微克时，7.2±1.7 mIU/ml；10微克时，15.7±3.4 mIU/ml；50微克时，37.7±9.6 mIU/ml；250微克时，47.8±11.2 mIU/ml；500微克时，49.3±11.2 mIU/ml。对每个剂量的平均FSH变化量分别为：2微克时，0.5±0.2 mIU/ml；10微克时，0.8±0.2 mIU/ml；50微克时，1.2±0.3 mIU/ml；250微克时，2.0±0.3 mIU/ml；500微克时，2.4±0.4 mIU/ml。由于对250微克剂量的LH和FSH反应均显著大于较小剂量时的反应，但对500微克剂量的反应并未进一步增大，因此250微克被用作后续研究的标准测试剂量。通过对42名精心挑选的正常男性静脉推注250微克剂量的GnRH来测试年龄的影响，这42名男性分三个年龄组，每组14人，年龄分别为20至39岁、40至59岁和60至79岁。可透析血清睾酮水平随年龄增长而下降，从21.7±4.6降至16.0±4.9再降至14.3±3.8 ng/dl（平均±标准差），基础血清LH水平略有升高，从7.4±3.4升至9.6±3.1再升至10.8±3.2 mIU/ml，这表明随着年龄增长，发生了轻度原发性睾丸间质细胞功能减退。尽管基础LH水平升高，但随着年龄增长，对GnRH反应的平均最大LH变化量并未相应增加。实际上，三个年龄组的最大LH变化量/基础LH比值从6.8降至5.1再降至3.3。基础血清FSH水平未随年龄增长而变化，但平均FSH变化量从3.0±0.5降至2.9±1.1±0.3 mIU/ml。这些数据表明，在男性衰老过程中，对合成GnRH的LH和FSH反应降低，同时存在一些原发性睾丸间质细胞功能减退。