Cope G, Nayyar P, Holder R, Brock G, Chapple I
Wolfson Applied Technology Laboratory, School of Dentistry, The University of Birmingham, UK.
Ann Clin Biochem. 2000 Sep;37 ( Pt 5):666-73. doi: 10.1258/0004563001899717.
Smoking is a major risk factor for periodontal disease, causing bone and tooth loss. Effective management of many oral conditions requires accurate information about smoking. We describe a disposable, 10-min, near-patient saliva test to monitor cigarette smoking. A plastic device contains the dried reagents to measure nicotine and its metabolites by a colorimetric assay. The device can be used to give a semi-quantitative assessment of tobacco consumption by observing a colour change and comparing it to a reference chart. Alternatively, the test can be quantified by measuring the light absorbance with a colorimeter and determining the concentration of nicotine metabolites by reference to a cotinine standard. This method correlates with reported daily cigarette consumption (r=0.62, P<0.0001) and nicotine yield of cigarettes smoked (r=0.35, P<0.01) and compares favourably with cotinine, as measured independently by radioimmunoassay (r=0.57, P<0.005). The method provides a simple-to-use, inexpensive way of monitoring tobacco consumption in dental surgeries.
吸烟是牙周病的主要风险因素，会导致骨质流失和牙齿脱落。有效管理多种口腔疾病需要有关吸烟情况的准确信息。我们描述了一种用于监测吸烟情况的一次性、耗时10分钟的即时唾液检测方法。一个塑料装置装有干燥试剂，通过比色法测量尼古丁及其代谢物。该装置可通过观察颜色变化并与参考图表比较，对烟草消耗量进行半定量评估。或者，可通过用比色计测量吸光度并参照可替宁标准确定尼古丁代谢物浓度来对检测进行量化。该方法与报告的每日吸烟量相关（r = 0.62，P < 0.0001），与所吸香烟的尼古丁含量相关（r = 0.35，P < 0.01），并且与通过放射免疫分析法独立测量的可替宁相比具有优势（r = 0.57，P < 0.005）。该方法为在牙科手术中监测烟草消耗量提供了一种简单易用且成本低廉的方式。
