Cope G, Nayyar P, Holder R, Gibbons J, Bunce R
Wolfson Applied Technology Laboratory, University of Birmingham, Queen Elizabeth Medical Center, UK.
Clin Chim Acta. 1996 Dec 30;256(2):135-49. doi: 10.1016/s0009-8981(96)06417-0.
We describe a disposable, near-patient urine test to monitor cigarette smoking. A plastic device contains the sealed dried reagents to measure nicotine and its metabolites, by a colorimetric assay. The device can be used to give a qualitative assessment of tobacco consumption, simply by observing a colour change. Alternatively, the test can be quantified by measuring the light absorbance with a simple colorimeter, and a concentration of nicotinic metabolites obtained with reference to a cotinine standard. A correction factor for the concentration of the urine sample, based on light absorbance, allows the result to be expressed as a ratio to urine concentration. This method correlates with reported daily cigarette consumption (r = 0.69, p < 0.0001) and compares favourably with cotinine, as measured by gas chromatography (r = 0.89, p < 0.0001). The method provides a simple-to-use, inexpensive way to monitoring tobacco consumption in extralaboratory situations.
我们描述了一种用于监测吸烟情况的一次性即时尿液检测方法。一种塑料装置包含密封的干燥试剂，通过比色法来测量尼古丁及其代谢物。该装置只需通过观察颜色变化，就能对烟草消费进行定性评估。或者，可使用简单的比色计测量吸光度，参照可替宁标准得出烟碱代谢物的浓度，从而对检测进行量化。基于吸光度的尿液样本浓度校正因子，可使结果表示为与尿液浓度的比值。该方法与报告的每日吸烟量相关（r = 0.69，p < 0.0001），与气相色谱法测量的可替宁相比效果良好（r = 0.89，p < 0.0001）。该方法提供了一种在实验室外环境中监测烟草消费的简单易用且经济实惠的方式。
