Kritzik M R, Krahl T, Good A, Krakowski M, St-Onge L, Gruss P, Wright C, Sarvetnick N
Department of Immunology, The Scripps Research Institute, La Jolla, CA 92037, USA.
Mol Cell Endocrinol. 2000 Jun;164(1-2):99-107. doi: 10.1016/s0303-7207(00)00234-3.
Recent studies by a number of laboratories have identified transcription factors that are involved in pancreatic development. Indeed, marked abnormalities in pancreatic development result from deficiencies in these molecules, which include, among others, PDX-1, islet-1 (Isl-1), and Pax-6. These studies have prompted us to evaluate the expression of Isl-1 and Pax-6 in the pancreas of the interferon-gamma (IFNgamma) transgenic mouse, which exhibits new islet growth and expansion of ducts throughout the life of the animal. We have previously demonstrated that PDX-1 is strikingly expressed in the ducts of the IFNgamma transgenic mouse. This latter observation compelled us to examine expression of hepatocyte nuclear factor-3beta (HNF3beta), which mediates PDX-1 gene transcription, in the IFNgamma transgenic pancreas as well. As a result of these studies, we now demonstrate marked expression of these transcription factors in the pancreatic ducts of IFNgamma transgenic mice. These data suggest a role for these transcription factors during pancreatic regeneration in the IFNgamma transgenic mouse.
最近多个实验室的研究已经鉴定出参与胰腺发育的转录因子。事实上，胰腺发育中的显著异常是由这些分子的缺陷导致的，其中包括PDX-1、胰岛-1（Isl-1）和Pax-6等。这些研究促使我们评估干扰素-γ（IFNγ）转基因小鼠胰腺中Isl-1和Pax-6的表达，该转基因小鼠在其整个生命过程中表现出新的胰岛生长和导管扩张。我们之前已经证明PDX-1在IFNγ转基因小鼠的导管中显著表达。后一观察结果促使我们也检测在IFNγ转基因胰腺中介导PDX-1基因转录的肝细胞核因子-3β（HNF3β）的表达。作为这些研究的结果，我们现在证明这些转录因子在IFNγ转基因小鼠的胰腺导管中显著表达。这些数据表明这些转录因子在IFNγ转基因小鼠胰腺再生过程中发挥作用。
