人甲状腺髓样癌细胞系中修饰的降钙素/降钙素基因相关肽启动子的高水平、组织特异性表达。

High level, tissue-specific expression of a modified calcitonin/calcitonin gene-related peptide promoter in a human medullary thyroid carcinoma cell line.

作者信息

Messina M, Yu D M, Learoyd D L, Both G W, Molloy P L, Robinson B G

机构信息

Department of Cancer Genetics, Kolling Institute of Medical Research, Royal North Shore Hospital, St. Leonards, NSW, Australia.

出版信息

Mol Cell Endocrinol. 2000 Jun;164(1-2):219-24. doi: 10.1016/s0303-7207(00)00204-5.

DOI:10.1016/s0303-7207(00)00204-5

PMID:11026573

Abstract

The efficient and high level expression of therapeutic genes in target cells is critical for effective gene therapy. We have developed a novel promoter by utilizing tandem repeats of a tissue-specific regulatory element from the calcitonin/calcitonin gene-related peptide (CT/CGRP) gene placed in close proximity to a basal promoter, thereby removing interstitial sequences. This promoter drives expression of reporter genes at much higher levels than the natural promoter while significantly improving specificity in thyroid C cells.

摘要

治疗性基因在靶细胞中的高效高水平表达对于有效的基因治疗至关重要。我们通过利用降钙素/降钙素基因相关肽（CT/CGRP）基因的组织特异性调控元件的串联重复序列，将其置于与基础启动子紧密相邻的位置，从而去除间隔序列，开发出了一种新型启动子。该启动子驱动报告基因的表达水平远高于天然启动子，同时显著提高了在甲状腺C细胞中的特异性。