Lambrechts A, Braun A, Jonckheere V, Aszodi A, Lanier L M, Robbens J, Van Colen I, Vandekerckhove J, Fässler R, Ampe C
Department of Biochemistry, Ghent University and Flanders Interuniversity Institute for Biotechnology, 9000 Ghent, Belgium.
Mol Cell Biol. 2000 Nov;20(21):8209-19. doi: 10.1128/MCB.20.21.8209-8219.2000.
We deduced the structure of the mouse profilin II gene. It contains five exons that can generate four different transcripts by alternative splicing. Two transcripts encode different profilin II isoforms (designated IIa and IIb) that have similar affinities for actin but different affinities for polyphosphoinositides and proline-rich sequences. Profilins IIa and IIb are also present in humans, suggesting that all mammals have three profilin isoforms. Profilin I is the major form in all tissues, except in the brain, where profilin IIa is most abundant. Profilin IIb appears to be a minor form, and its expression is restricted to a limited number of tissues, indicating that the alternative splicing is tightly regulated. Western blotting and whole-mount in situ hybridization show that, in contrast to the expression of profilin I, the expression level of profilin IIa is developmentally regulated. In situ hybridization of adult brain sections reveals overlapping expression patterns of profilins I and IIa.
我们推导了小鼠丝切蛋白II基因的结构。它包含五个外显子，可通过可变剪接产生四种不同的转录本。两种转录本编码不同的丝切蛋白II同工型（命名为IIa和IIb），它们对肌动蛋白具有相似的亲和力，但对多磷酸肌醇和富含脯氨酸的序列具有不同的亲和力。丝切蛋白IIa和IIb在人类中也存在，这表明所有哺乳动物都有三种丝切蛋白同工型。除了在大脑中丝切蛋白IIa含量最为丰富外，丝切蛋白I是所有组织中的主要形式。丝切蛋白IIb似乎是次要形式，其表达仅限于有限数量的组织，这表明可变剪接受严格调控。蛋白质免疫印迹和整体原位杂交表明，与丝切蛋白I的表达相反，丝切蛋白IIa的表达水平受发育调控。成人大脑切片的原位杂交显示丝切蛋白I和IIa的表达模式重叠。
