刚地弓形虫抗原的菌株和阶段特异性变异

Strain and stage specific variation in Toxoplasma gondii antigens.

作者信息

Appleford P J, Smith J E

机构信息

School of Biology, Miall Building, University of Leeds, Leeds, LS2 9JT, UK.

出版信息

Int J Parasitol. 2000 Oct;30(11):1187-91. doi: 10.1016/s0020-7519(00)00109-0.

DOI:10.1016/s0020-7519(00)00109-0

PMID:11027786

Abstract

The antigenic profile of virulent (RH, ENT, Martin) and avirulent (RRA, DEG, ME49) Toxoplasma strains was compared directly by western blotting using a panel of immune mouse sera. Dominant antigens of approximate MR 30-33, 21 and 25 x 10(3) were common to tachyzoites of all strains, however, there were significant quantitative and qualitative differences in the antigen profiles, indicating a moderate degree of strain specific polymorphism in tachyzoite antigens. We found no specific association between antigenic variation and strain virulence. Comparison of tachyzoite and bradyzoite antigens from homologous strains (RRA, DEG, ME49) confirmed the existence of stage specific antigens and demonstrated a conserved antigen profile among bradyzoites.

摘要

使用一组免疫小鼠血清，通过蛋白质印迹法直接比较了强毒株（RH、ENT、Martin）和无毒株（RRA、DEG、ME49）弓形虫菌株的抗原谱。所有菌株速殖子都有大约30 - 33、21和25×10³的主要抗原，但抗原谱存在显著的数量和质量差异，表明速殖子抗原存在中等程度的菌株特异性多态性。我们未发现抗原变异与菌株毒力之间存在特定关联。对同源菌株（RRA、DEG、ME49）的速殖子和缓殖子抗原进行比较，证实了阶段特异性抗原的存在，并显示缓殖子之间存在保守的抗原谱。