Lämmerhofer M, Svec F, Fréchet J M
Department of Chemistry, University of California, Berkeley 94720-1460, USA.
Anal Chem. 2000 Oct 1;72(19):4623-8. doi: 10.1021/ac000323d.
The effect of chromatographic conditions on the performance of chiral monolithic poly(O-[2-(methacryloyloxy)-ethylcarbamoyl]-10,11-dihydroqui nidine-co-ethylene dimethacrylate-co-2-hydroxyethyl methacrylate) columns in the capillary electrochromatography of enantiomers has been studied. The flow velocity was found to be proportional to the pore size of the monolith and both the pH and the composition of the mobile phase. The length of both open and monolithic segments of the capillary column was found to exert a substantial effect on the run times. The use of monoliths as short as 8.5 cm and the "short-end" injection technique enabled the separations to be achieved in approximately 5 min despite the high retentitivity of the quinidine selector. Very high column efficiencies of close to 250000 plates/m and good selectivities were achieved for the separations of numerous enantiomers using the chiral monolithic capillaries with the optimized chromatographic conditions.
研究了色谱条件对用于对映体毛细管电色谱的手性整体式聚（O-[2-(甲基丙烯酰氧基)-乙基氨基甲酰基]-10,11-二氢奎尼丁-共-乙二醇二甲基丙烯酸酯-共-甲基丙烯酸2-羟乙酯）柱性能的影响。发现流速与整体柱的孔径以及流动相的pH值和组成成正比。发现毛细管柱开放段和整体段的长度对运行时间有显著影响。尽管奎尼丁选择剂的保留性很高，但使用短至8.5 cm的整体柱和“短端”进样技术，仍能在约5分钟内实现分离。在优化的色谱条件下，使用手性整体式毛细管对多种对映体进行分离时，实现了接近250000塔板/米的非常高的柱效和良好的选择性。
