Peters E C, Petro M, Svec F, Fréchet J M
Department of Chemistry, University of California, Berkeley 94720-1460, USA.
Anal Chem. 1998 Jun 1;70(11):2296-302. doi: 10.1021/ac9713520.
The effect of chromatographic conditions on the performance of monolithic poly(butyl methacrylate-co-ethylene dimethacrylate-co-2-acrylamido-2-methyl-1-propanesulfonic acid) columns in capillary electrochromatography has been studied. The flow velocity was found to be proportional to the strength of the electric field and both the pH and the composition of the mobile phase. A column efficiency of 120,000 plates/m at the optimum flow velocity of 1.5 cm/min is achieved for all the monolithic capillary columns of identical composition and porosity, regardless of their length, which varied from 30 to 120 cm. The polymeric separation medium exhibits retention and selectivity properties similar to those of typical ODS packings for reversed-phase chromatography. In addition to the "classical" use of monolithic capillary columns for the electrochromatographic separation of small molecules in reversed-phase mode, larger styrene oligomers were also separated under isocratic elution conditions. In addition, the electroosmotically driven size exclusion chromatography of polystyrene standards with molecular weights up to 10(6) has been demonstrated for the first time.
研究了色谱条件对整体式聚（甲基丙烯酸丁酯 - 二甲基丙烯酸乙二醇酯 - 2 - 丙烯酰胺基 - 2 - 甲基 - 1 - 丙烷磺酸）毛细管电色谱柱性能的影响。发现流速与电场强度以及流动相的pH值和组成成正比。对于所有组成和孔隙率相同的整体式毛细管柱，无论其长度如何变化（从30厘米到120厘米），在1.5厘米/分钟的最佳流速下，柱效均达到120,000塔板/米。该聚合物分离介质表现出与典型反相色谱ODS填料相似的保留和选择性特性。除了整体式毛细管柱在反相模式下用于小分子电色谱分离的“经典”用途外，在等度洗脱条件下还分离了较大的苯乙烯低聚物。此外，首次展示了对分子量高达10(6)的聚苯乙烯标准品进行电渗驱动的尺寸排阻色谱分析。
