Eckhardt T, Leisinger T
Mol Gen Genet. 1975 Jun 19;138(3):225-32. doi: 10.1007/BF00269349.
Mutants with a feedback resistant N-acetylglutamate synthase have been isolated from a proA/B, argD, argR strain by screening for proline excretion on minimal medium with arginine. The feedback resistant character of three mutants was transduced into an argA (N-acetylglutamate synthase negative) strain. It was cotransducible with argA at a frequency of greater than 99%. N-acetylglutamate synthase extracted from the three mutants was approximately one hundred times less sensitive to L-arginine than the enzyme from the feedback sensitive parent strain.
通过在含有精氨酸的基本培养基上筛选脯氨酸排泄情况，从proA/B、argD、argR菌株中分离出了具有反馈抗性的N-乙酰谷氨酸合酶突变体。三个突变体的反馈抗性特征被转导到argA（N-乙酰谷氨酸合酶阴性）菌株中。它与argA的共转导频率大于99%。从这三个突变体中提取的N-乙酰谷氨酸合酶对L-精氨酸的敏感性比来自反馈敏感亲本菌株的酶低约一百倍。
