鳗弧菌铁摄取调节蛋白（Fur）的DNA和金属结合特性表征揭示了一个结构锌离子（Zn²⁺）的保守性。
Characterization of the DNA- and metal-binding properties of Vibrio anguillarum fur reveals conservation of a structural Zn(2+) ion.
作者信息
Zheleznova E E, Crosa J H, Brennan R G
机构信息
Department of Biochemistry and Molecular Biology, Oregon Health Sciences University, Portland, Oregon 97201-3098, USA.
出版信息
J Bacteriol. 2000 Nov;182(21):6264-7. doi: 10.1128/JB.182.21.6264-6267.2000.
Abstract
The ferric uptake regulator, Fur, represses iron uptake and siderophore biosynthetic genes under iron-replete conditions. Here we report in vitro solution studies on Vibrio anguillarum Fur binding to the consensus 19-bp Escherichia coli iron box in the presence of several divalent metals. We found that V. anguillarum Fur binds the iron box in the presence of Mn(2+), Co(2+), Cd(2+), and to a lesser extent Ni(2+) but, unlike E. coli Fur, not in the presence of Zn(2+). We also found that V. anguillarum Fur contains a structural zinc ion that is necessary yet alone is insufficient for DNA binding.
摘要
铁摄取调节蛋白（Fur）在铁充足的条件下会抑制铁摄取和铁载体生物合成基因。在此，我们报告了在几种二价金属存在的情况下，对鳗弧菌Fur与大肠杆菌共有19碱基对铁框结合的体外溶液研究。我们发现，鳗弧菌Fur在存在锰离子（Mn²⁺）、钴离子（Co²⁺）、镉离子（Cd²⁺）时能结合铁框，在一定程度上镍离子（Ni²⁺）存在时也能结合，但与大肠杆菌Fur不同的是，在锌离子（Zn²⁺）存在时不能结合。我们还发现，鳗弧菌Fur含有一个结构锌离子，它对于DNA结合是必要的，但仅靠它是不够的。
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