Beebe N W, Cooper R D, Morrison D A, Ellis J T
Molecular Parasitology Unit, Department of Cell and Molecular Biology, University of Technology, Sydney, NSW, Australia.
Insect Mol Biol. 2000 Oct;9(5):515-20. doi: 10.1046/j.1365-2583.2000.00211.x.
A phylogenetic study, based on maximum parsimony, of ten species in the Anopheles punctulatus group of malaria vectors from the south-west Pacific was performed using structural and similarity-based DNA sequence alignments of the nuclear small ribosomal subunit (SSU = 18S). The structural alignment proved to be more informative than a computer generated similarity-based alignment. Analyses involving the full structural sequence alignment (2169 bp) and the helical regions (1547 bp) resolved a single tree of the same topology, while analyses using the similarity based alignment could not resolve the group. Studies on the three structural domains of the nuclear rDNA SSU identified domain 2 (769 bp) as the only region informative at the sibling-species level and resulted in the same tree as the full structural sequence and helical regions. The main conclusions of these studies were that the An. punctulatus group formed two clades: a Farauti clade containing members displaying an all black scaled proboscis (An. farauti 1-3 and 5-7) and a Punctulatus clade containing members that display some degree of white scaling on the proboscis (An. farauti 4, An. punctulatus and An. species near punctulatus). Anopheles koliensis can display either proboscis morphology and was positioned basal to the Farauti Clade. These results do not fully concord with those derived from the mitochondrial COII gene.
基于最大简约法，对来自西南太平洋的按蚊属致倦库蚊组中的10个物种进行了系统发育研究，使用核小核糖体亚基（SSU = 18S）的基于结构和相似性的DNA序列比对。结果表明，结构比对比计算机生成的基于相似性的比对更具信息性。涉及完整结构序列比对（2169 bp）和螺旋区域（1547 bp）的分析解析出一棵具有相同拓扑结构的单一树，而使用基于相似性的比对的分析无法解析该组。对核rDNA SSU的三个结构域的研究确定结构域2（769 bp）是在姐妹物种水平上唯一具有信息性的区域，并产生了与完整结构序列和螺旋区域相同的树。这些研究的主要结论是，致倦库蚊组形成了两个进化枝：一个法拉乌蒂进化枝，包含显示全黑鳞片喙的成员（致倦库蚊1-3和5-7），以及一个点斑进化枝，包含在喙上显示一定程度白色鳞片的成员（致倦库蚊4、点斑按蚊和近点斑按蚊物种）。科利按蚊可以表现出两种喙形态，并位于法拉乌蒂进化枝的基部。这些结果与来自线粒体COII基因的结果并不完全一致。
