Laconi S, Greco M, Pellegrini-Bettoli P, Pani P, Laconi E, Rais M
Dipartimento di Scienze Bio-Mediche e Biotecnologie, Università di Cagliari.
Pathologica. 2000 Aug;92(4):236-40.
Human Papillomavirus (HPV) infection is considered an important risk factor for the development of cervical carcinoma. The aim of this work was to detect and genotype HPV DNA in cervical lesions from our Province.
HPV DNA was amplified by polymerase chain reaction (PCR) and genotyped by restriction fragment length polymorphism (RFLP) analysis. A total of 101 biopsies (43 koilocytic atypias, 20 CIN1, 19 CIN2, 17 CIN3 and 2 squamous carcinomas) were analyzed.
HPV DNA was found in 41.8% of koilocytic atypias, in 95.0% of CIN1 and 100% of CIN2 and higher grade lesions. Only high risk genotypes were found in CIN2-3 and invasive carcinomas. HPV 16 was the most prevalent type in both CIN1 and CIN2-3 and the only HPV type found in situ and invasive carcinomas. HPV type 51 was found in 21.0% of CIN1 but it was rare in CIN2 and absent in more advanced lesions.
人乳头瘤病毒（HPV）感染被认为是宫颈癌发生的重要危险因素。本研究的目的是检测我省宫颈病变中HPV DNA并进行基因分型。
采用聚合酶链反应（PCR）扩增HPV DNA，并通过限制性片段长度多态性（RFLP）分析进行基因分型。共分析了101份活检标本（43例挖空细胞异型增生、20例CIN1、19例CIN2、17例CIN3和2例鳞状细胞癌）。
在41.8%的挖空细胞异型增生、95.0%的CIN1以及100%的CIN2和更高级别病变中发现了HPV DNA。在CIN2 - 3和浸润性癌中仅发现高危基因型。HPV-16是CIN1和CIN2 - 3中最常见的类型，也是原位癌和浸润性癌中唯一发现的HPV类型。HPV-51在21.0%的CIN1中被发现，但在CIN2中少见，在更晚期病变中未出现。
