曼谷地区正常宫颈、上皮内瘤变及宫颈癌中人乳头瘤病毒DNA的检测与分型

Detection and typing of human papilloma virus DNAs in normal cervix, intraepithelial neoplasia and cervical cancer in Bangkok.

作者信息

Siritantikorn S, Laiwejpithaya S, Siripanyaphinyo U, Auewarakul P, Yenchitsomanus P, Thakernpol K, Wasi C

机构信息

Department of Microbiology, Faculty of Medicine Siriraj Hospital, Bangkok, Thailand.

出版信息

Southeast Asian J Trop Med Public Health. 1997 Dec;28(4):707-10.

PMID:9656389

Abstract

We detected and typed HPV-DNA by polymerase chain reaction (PCR) in cervico-vaginal lavages of 102 women with normal cervical cytology, 57 patients with cervical intraepithelial neoplasia (CIN), and 23 cervical cancer patients. HPV-DNA detection and typing by in situ hybridization were also performed in cervical biopsies from CIN lesions and cancers. Five percent of women with normal cervical cytology, 46% of CIN, and 61% of cervical cancer were positive for HPV-DNA. Of CIN cases with positive HPV-DNA, 69, 15, 8, 4 and 4% were HPV-16, -33, -18, -11 and -16/33 respectively. Of cervical cancer cases with positive HPV-DNA, 86% were HPV-16, 7% were HPV-16/33, 7% were HPV-18/31. HPV typing was performed in biopsies from 37 CIN and 18 cervical cancers by in situ hybridization. By this method, 38% of CIN were HPV-DNA positive, of which 71% were HPV-16 and 7% were each of HPV-11, -18, -31 and -33. Thirty-nine percent of cervical cancers were positive, of which 71% and 29% were HPV-16 and HPV-16/18 respectively.

摘要

我们采用聚合酶链反应（PCR）对102例宫颈细胞学正常的女性、57例宫颈上皮内瘤变（CIN）患者及23例宫颈癌患者的宫颈阴道灌洗液进行HPV-DNA检测及分型。同时，对CIN病变及癌组织的宫颈活检标本进行原位杂交HPV-DNA检测及分型。宫颈细胞学正常的女性中5%、CIN患者中46%、宫颈癌患者中61%的HPV-DNA呈阳性。HPV-DNA阳性的CIN病例中，HPV-16、-33、-18、-11及-16/33分别占69%、15%、8%、4%及4%。HPV-DNA阳性的宫颈癌病例中，HPV-16占86%，HPV-16/33占7%，HPV-18/31占7%。通过原位杂交对37例CIN及18例宫颈癌的活检标本进行HPV分型。采用该方法，38%的CIN为HPV-DNA阳性，其中71%为HPV-16，HPV-11、-18、-31及-33各占7%。39%的宫颈癌呈阳性，其中HPV-16及HPV-16/18分别占71%及29%。