Greeley G H, Allen M B, Mahesh V B
Neuroendocrinology. 1975;18(3):233-41. doi: 10.1159/000122402.
The objective of this study was to determine the influence of estradiol-17beta and/or progesterone on gonadotropon secretion at the level of the pituitary. Female rats in which the hypothalamo-hypophyseal connections had been permanently interrupted after castration served as the experimental model in which the effect of estradiol and/or progesterone on LH-RH-induced gonadotropin release was examined. In out experimental animals, LH secretion was readily activated by LH-RH administration. LH release was greatly augmented by the prior administration of estradiol benzoate (1 mug/kg b.w./day). Progesterone (5 mg/day) in the absence of estradiol did not modify the 10-min responese to LH-RH but reduced the enhancement of LH secretion caused by estradiol pretreatment. Our findings suggest that estradiol potentiated the releasing effect of LH-RH at the level of the gonadotroph, whereas progesterone interfered with the potentiation effect. Plasma levels of FSH were not significantly elevated above the basal value by the administration of LH-RH alone, or in combination with estradiol and/or progesterone.
本研究的目的是确定17β-雌二醇和/或孕酮对垂体水平促性腺激素分泌的影响。下丘脑-垂体连接在去势后已被永久中断的雌性大鼠作为实验模型，用于研究雌二醇和/或孕酮对促性腺激素释放激素（LH-RH）诱导的促性腺激素释放的影响。在我们的实验动物中，给予LH-RH可轻易激活LH分泌。预先给予苯甲酸雌二醇（1微克/千克体重/天）可极大增强LH释放。在无雌二醇情况下，孕酮（5毫克/天）不会改变对LH-RH的10分钟反应，但会降低雌二醇预处理引起的LH分泌增强。我们的研究结果表明，雌二醇在促性腺激素细胞水平增强了LH-RH的释放作用，而孕酮则干扰了这种增强作用。单独给予LH-RH，或与雌二醇和/或孕酮联合给予，均未使血浆FSH水平显著高于基础值。
