Kobayashi R, Harada A, Kotani M, Yamada T, Wakabayashi K
Horm Metab Res. 1978 May;10(3):237-42. doi: 10.1055/s-0028-1093443.
In an attempt to study the site and mechanism of action of estrogen in producing positive feedback control, porcine anterior pituitary slices were incubated in vitro in the presence of estradiol benzoate (EB). EB elevated pituitary cyclic AMP concentration within 5 min and augmented pituitary release of luteinizing hormone (LH). The magnitude of increase of cyclic AMP and LH release was related to the doses of EB used. Also, luteinizing hormone releasing hormone (LH-RH) elevated pituitary cyclic AMP concentration and stimulated pituitary release of LH. The magnitude of increase of cyclic AMP and LH release was inversely related to the doses of LH-RH used. EB and LH-RH were additive in increasing cyclic AMP. Progesterone and clomiphene citrate interfered with an increase of pituitary cyclic AMP produced by EB, but did not significantly affect the basal level of pituitary cyclic AMP. Testosterone propionate, human chorionic gonadotropin and hexestrol were without effect on either basal or stimulated level of pituitary cyclic AMP. Since cyclic AMP and dibutyryl cyclic AMP (DBC) stimulated LH release, it is suggested that EB directly stimulates the release of LH by augmenting cyclic AMP synthesis in the anterior pituitary.
为了研究雌激素产生正反馈控制的作用部位和机制，将猪垂体前叶切片在苯甲酸雌二醇（EB）存在的情况下进行体外培养。EB在5分钟内升高了垂体环磷酸腺苷（cAMP）浓度，并增加了垂体促黄体生成素（LH）的释放。cAMP和LH释放增加的幅度与所用EB的剂量有关。此外，促黄体生成素释放激素（LH-RH）升高了垂体cAMP浓度，并刺激了垂体LH的释放。cAMP和LH释放增加的幅度与所用LH-RH的剂量呈负相关。EB和LH-RH在增加cAMP方面具有相加作用。孕酮和枸橼酸氯米芬干扰了EB引起的垂体cAMP增加，但对垂体cAMP的基础水平没有显著影响。丙酸睾酮、人绒毛膜促性腺激素和己烯雌酚对垂体cAMP的基础水平或刺激水平均无影响。由于cAMP和二丁酰环磷酸腺苷（DBC）刺激了LH释放，提示EB通过增强垂体前叶cAMP合成直接刺激LH释放。
