Schröder S, Hain M, Sterflinger K
Department of Geomicrobiology, Institute of Chemistry and Biology of the Marine Environment, Carl von Ossietzky-University, Oldenburg, Germany.
Int Microbiol. 2000 Sep;3(3):183-6.
We used digoxigenin-labeled probes for in situ hybridization of hyphomycetes to replace the commonly used fluorescent proof of probe binding by a colorimetric reaction. The resulting blue-purple, intracellular precipitate could be easily detected by light microscopy, and thus presented a promising method to overcome autofluorescence of fungal material and substratum. Optimal cell fixation and permeabilization procedures, as well as hybridization conditions were developed on the example of two different hyphomycetes: Phialophora sp. and Cartapip, a colorless mutant of Ophiostoma piliferum (Agra Sol).
