Takenaka M, Yamaoka S, Hanajiri T, Shimizu-Ueda Y, Yamato K T, Fukuzawa H, Ohyama K
Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University, Japan.
Transgenic Res. 2000 Jun;9(3):179-85. doi: 10.1023/a:1008963410465.
Thalli of the haploid liverwort Marchantial polymorpha were successfully used for direct particle bombardment with plasmid pMT, which carries a hygromycin phosphotransferase gene (hpt) controlled by the CaMV 35S promoter and the NOS polyadenylation region. Hygromycin-resistant cell masses arose from the thallus surface and developed directly into hygromycin-resistant thalli. Southern blot analyses indicated that these thalli carried at least 1-4 copies of the hpt gene, which were stably transmitted to their asexual thallus progenies via gemma propagation for three generations. This transformation and direct plant regeneration protocol is expected to be a valuable tool for the molecular analysis of this lower land plant.
单倍体地钱多形钱苔的叶状体成功用于用携带由花椰菜花叶病毒35S启动子和胭脂碱合成酶聚腺苷酸化区域控制的潮霉素磷酸转移酶基因（hpt）的质粒pMT进行直接粒子轰击。潮霉素抗性细胞团从叶状体表面产生，并直接发育成潮霉素抗性叶状体。Southern印迹分析表明，这些叶状体携带至少1 - 4个拷贝的hpt基因，这些基因通过芽孢繁殖稳定地传递给它们的无性叶状体后代三代。这种转化和直接植物再生方案有望成为这种低等陆生植物分子分析的有价值工具。
