Nakamura H
Tophoku National Agricultural Experiment Station, Morioka, Iwate, Japan.
J Agric Food Chem. 2000 Jul;48(7):2648-52. doi: 10.1021/jf9912654.
To reveal the high-molecular-weight (1-1MW) glutenin subunit composition, the seed storage proteins of 40 Japanese wheat (Triticum aestivum) lines were fractionated by sodium dodecyl sulfate- polyacrylamide gel electrophoresis to determine their HMW glutenin subunit composition. These were identified by comparison of subunit mobility with that previously found in hexaploid wheat. Twelve different, major glutenin HMW subunits were identified. Each line contained three to five subunits, and 11 different glutenin subunit patterns were observed for 11 alleles in Japanese lines. The Glu-1 quality scores were not particularly high for most of the Japanese wheats in the southern part of Japan (Kyushu district). However, the Glu-1 quality scores of several wheat lines in the Hokkaido area (north Japan) were high. South Japanese wheat lines showed specialty allelic variation in the glutenin HMW 145 kfla subunit, different from those in non-Japanese hexaploid wheats.
为揭示高分子量（HMW）谷蛋白亚基组成，对40个日本小麦（普通小麦）品系的种子贮藏蛋白进行十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分级分离，以确定其HMW谷蛋白亚基组成。通过将亚基迁移率与先前在六倍体小麦中发现的迁移率进行比较来鉴定这些亚基。鉴定出12种不同的主要谷蛋白HMW亚基。每个品系包含3至5个亚基，在日本品系的11个等位基因中观察到11种不同的谷蛋白亚基模式。日本南部（九州地区）的大多数日本小麦的Glu-1品质评分并不特别高。然而，北海道地区（日本北部）的几个小麦品系的Glu-1品质评分较高。日本南部的小麦品系在谷蛋白HMW 145 kDa亚基中表现出特殊的等位基因变异，这与非日本六倍体小麦中的不同。
